AC   MF_01102;
DC   Protein; auto
TR   HAMAP; MF_01102; -; 1; level=0
XX
Names: MnmC
XX
ID   MNMC
DE   RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC;
DE            Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein;
DE   Includes:
DE     RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase;
DE              EC=2.1.1.61;
DE   Includes:
DE     RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase;
DE              EC=1.5.-.-;
GN   Name=mnmC;
XX
CC   -!- FUNCTION: Catalyzes the last two steps in the biosynthesis of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position
CC       (U34) in tRNA. Catalyzes the FAD-dependent demodification of
CC       cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl
CC       group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form
CC       mnm(5)s(2)U34.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L-
CC         methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) +
CC         S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:19569, Rhea:RHEA-
CC         COMP:10195, Rhea:RHEA-COMP:10197, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74454,
CC         ChEBI:CHEBI:74455; EC=2.1.1.61;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the methyltransferase
CC       superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the DAO family.
XX
DR   Pfam; PF01266; DAO; 1; trigger=no
DR   Pfam; PF05430; Methyltransf_30; 1; trigger=no
DR   NCBIfam; TIGR03197; MnmC_Cterm; 1; trigger=no
XX
KW   Cytoplasm
KW   FAD
KW   Flavoprotein
KW   Methyltransferase
KW   Multifunctional enzyme
KW   Oxidoreductase
KW   S-adenosyl-L-methionine
KW   Transferase
KW   tRNA processing
XX
GO   GO:0050660; F:flavin adenine dinucleotide binding
GO   GO:0016491; F:oxidoreductase activity
GO   GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity
GO   GO:0002097; P:tRNA wobble base modification
GO   GO:0005737; C:cytoplasm
XX
FT   From: MNMC_ECOLI (P77182)
FT   REGION          Nter..245
FT                   /note="tRNA (mnm(5)s(2)U34)-methyltransferase"
FT   REGION          270..Cter
FT                   /note="FAD-dependent cmnm(5)s(2)U34 oxidoreductase"
XX
Size: 585-754;
Related: None;
Template: P77182;
Scope:
 Bacteria; Pseudomonadota
 Bacteria; Spirochaetota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.33 2023/06/01
//