AC MF_01120; DC Protein; auto TR HAMAP; MF_01120; -; 1; level=0 XX Names: LysK XX ID LYSK case DE RecName: Full=[LysW]-lysine/[LysW]-ornithine hydrolase; DE EC=3.5.1.130; else case and not DE RecName: Full=Putative [LysW]-lysine/[LysW]-ornithine hydrolase; DE EC=3.5.1.130; DE EC=3.5.1.132; else case DE RecName: Full=[LysW]-lysine hydrolase; DE EC=3.5.1.130; else case DE RecName: Full=Putative [LysW]-lysine hydrolase; DE EC=3.5.1.130; end case GN Name=lysK; XX case CC -!- FUNCTION: Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine CC and the release of L-ornithine from [LysW]-L-ornithine. else case CC -!- FUNCTION: Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L- CC glutamate + H2O = [amino-group carrier protein]-C-terminal-L- CC glutamate + L-lysine; Xref=Rhea:RHEA:48684, Rhea:RHEA-COMP:9693, CC Rhea:RHEA-COMP:9715, ChEBI:CHEBI:15377, ChEBI:CHEBI:32551, CC ChEBI:CHEBI:78525, ChEBI:CHEBI:78526; EC=3.5.1.130; case CC -!- CATALYTIC ACTIVITY: CC Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L- CC glutamate + H2O = [amino-group carrier protein]-C-terminal-L- CC glutamate + L-ornithine; Xref=Rhea:RHEA:52676, Rhea:RHEA-COMP:9693, CC Rhea:RHEA-COMP:13328, ChEBI:CHEBI:15377, ChEBI:CHEBI:46911, CC ChEBI:CHEBI:78525, ChEBI:CHEBI:136763; EC=3.5.1.132; end case CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Name=Co(2+); Xref=ChEBI:CHEBI:48828; CC Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.; CC -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via AAA CC pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5. case CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis. end case CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the peptidase M20A family. LysK subfamily. XX DR Pfam; PF01546; Peptidase_M20; 1; trigger=no DR NCBIfam; TIGR01902; DapE-lys-deAc; 1; trigger=no DR PROSITE; PS00758; ARGE_DAPE_CPG2_1; 1; trigger=no DR PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1; trigger=no XX KW Amino-acid biosynthesis KW Cytoplasm KW Hydrolase KW Lysine biosynthesis case KW Arginine biosynthesis end case KW Metal-binding KW Cobalt KW Zinc XX GO GO:0050897; F:cobalt ion binding GO GO:0008270; F:zinc ion binding GO GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides case GO GO:0042450; P:arginine biosynthetic process via ornithine end case GO GO:0019878; P:lysine biosynthetic process via aminoadipic acid GO GO:0005737; C:cytoplasm XX FT From: LYSK_THET2 (Q8VUS5) FT ACT_SITE 69 FT Condition: D FT ACT_SITE 124 FT /note="Proton acceptor" FT Condition: E FT BINDING 67 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT Condition: H FT BINDING 91 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT Condition: D FT BINDING 91 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT Condition: D FT BINDING 125 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT Condition: E FT BINDING 148 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="1" FT Condition: E FT BINDING 326 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="2" FT Condition: H XX Size: 320-425; Related: None; Template: Q8VUS5; Q4JAP7; Scope: Archaea Bacteria; Chloroflexota Bacteria; Deinococcota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.38 2023/06/01 //