AC   MF_01129;
DC   Protein; auto
TR   HAMAP; MF_01129; -; 1; level=0
XX
Names: PPase_energized_pump
XX
ID   HPPA
case <FTTag:Kdep>
DE   RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump;
DE            EC=7.2.3.1;
DE   AltName: Full=Membrane-bound sodium-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=Na(+)-PPase;
else case <FTTag:Kindep>
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump;
DE            EC=7.1.3.1;
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=H(+)-PPase;
else
DE   RecName: Full=Pyrophosphate-energized proton pump;
DE            EC=7.1.3.1;
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
DE            Short=H(+)-PPase;
end case
GN   Name=hppA;
XX
case <FTTag:Kdep>
CC   -!- FUNCTION: Sodium pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for Na(+) movement across the membrane.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2
CC         phosphate; Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474; EC=7.2.3.1;
else
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
end case
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
case <FTTag:Kdep>
CC   -!- ACTIVITY REGULATION: Requires K(+) for maximal activity.
end case
CC   -!- SUBUNIT: Homodimer.
case not defined <Property:Membrane> or <Property:Membrane=1>
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
else case <Property:Membrane=2>
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
end case
case <FTTag:Kdep>
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-stimulated subfamily.
else case <FTTag:Kindep>
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-insensitive subfamily.
else
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family.
end case
XX
DR   Pfam; PF03030; H_PPase; 1; trigger=no
DR   NCBIfam; TIGR01104; V_PPase; 1; trigger=no
DR   PIRSF; PIRSF001265; H+-PPase; 1; trigger=no
DR   General; Transmembrane; -; 13-18; trigger=yes
XX
case defined <Property:Membrane> and <Property:Membrane=2>
KW   Cell inner membrane
end case
KW   Cell membrane
KW   Ion transport
KW   Magnesium
KW   Membrane
case <FTTag:Kdep>
KW   Potassium
KW   Sodium
KW   Sodium transport
else
KW   Hydrogen ion transport
end case
KW   Translocase
KW   Transport
KW   Transmembrane
KW   Transmembrane helix
XX
GO   GO:0009678; F:diphosphate hydrolysis-driven proton transmembrane transporter activity
GO   GO:0004427; F:inorganic diphosphate phosphatase activity
GO   GO:0000287; F:magnesium ion binding
case <FTTag:Kdep>
GO   GO:0030955; F:potassium ion binding
GO   GO:0006814; P:sodium ion transport
else
GO   GO:1902600; P:proton transmembrane transport
end case
GO   GO:0005886; C:plasma membrane
XX
FT   From: HPPA_THEMA (Q9S5X0)
FT   SITE            495
FT                   /note="Determinant of potassium dependence"
FT   Tag: Kdep; Optional; Condition: A
FT   From: HPPA_PYRAE (Q8ZWI8)
FT   SITE            476
FT                   /note="Determinant of potassium independence"
FT   Tag: Kindep; Optional; Condition: K
XX
Size: 655-825;
Related: None;
Template: Q9S5X0; Q3AFC6; Q8ZWI8; Q8PYZ8;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: in METAC, METMA, MOOTA
Plasmid: None
Comments: There are two classes of pyrophosphate-energized pumps, differing in K(+) sensitivity (PubMed:17454298).
 Three members of the K(+)-stimulated subfamily have been characterized as sodium pumps (PubMed:17605473).
 Further characterization is needed to distinguish Na(+) transporters from H(+) transporters.
XX
# Revision 1.47 2023/07/28
//