AC MF_01137; DC Protein; auto TR HAMAP; MF_01137; -; 1; level=0 XX Names: CdhA XX ID ACDA DE RecName: Full=Acetyl-CoA decarbonylase/synthase complex subunit alpha; DE Short=ACDS complex subunit alpha; DE EC=1.2.7.4; DE AltName: Full=ACDS complex carbon monoxide dehydrogenase subunit alpha; DE Short=ACDS CODH subunit alpha; GN Name=cdhA; XX case CC -!- FUNCTION: Part of the ACDS complex that catalyzes the reversible CC cleavage of acetyl-CoA, allowing growth on acetate as sole source of CC carbon and energy. The alpha-epsilon subcomponent functions as a carbon CC monoxide dehydrogenase. end case case not CC -!- FUNCTION: Part of the ACDS complex that catalyzes the reversible CC cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The CC alpha-epsilon subcomponent functions as a carbon monoxide CC dehydrogenase. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 CC reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:21040, Rhea:RHEA- CC COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17245, CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.2.7.4; CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 7 [4Fe-4S] clusters per heterotetramer.; CC -!- COFACTOR: CC Name=[Ni-4Fe-4S] cluster; Xref=ChEBI:CHEBI:47739; CC Note=Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.; case CC -!- PATHWAY: One-carbon metabolism; methanogenesis from acetate. end case CC -!- SUBUNIT: Heterotetramer of two alpha and two epsilon subunits. The ACDS CC complex is made up of alpha, epsilon, beta, gamma and delta subunits CC with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)- CC (gamma(1)delta(1))(8). CC -!- DOMAIN: Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster CC C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. CC Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges CC the two subunits of the CODH dimer. Contains two additional 4Fe-4S CC clusters, dubbed E and F, that probably transport electrons from CC ferredoxin to the B cluster. CC -!- SIMILARITY: Belongs to the Ni-containing carbon monoxide dehydrogenase CC family. XX DR Pfam; PF00037; Fer4; 2; trigger=no DR Pfam; PF03063; Prismane; 1; trigger=no DR PRINTS; PR00353; 4FE4SFRDOXIN; 1; trigger=no DR NCBIfam; TIGR00314; CdhA; 1; trigger=no DR PROSITE; PS00198; 4FE4S_FER_1; 2; trigger=no DR PROSITE; PS51379; 4FE4S_FER_2; 2; trigger=yes XX case KW Methanogenesis end case KW Oxidoreductase KW Metal-binding case KW Nickel end case case or or or or KW Iron KW Iron-sulfur end case case or or or KW 4Fe-4S end case XX case or or or or GO GO:0005506; F:iron ion binding end case case GO GO:0016151; F:nickel cation binding end case GO GO:0018492; F:carbon-monoxide dehydrogenase (acceptor) activity case GO GO:0019385; P:methanogenesis, from acetate end case XX FT From: ACDA1_METBF (Q46G04) FT BINDING 73 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT /ligand_note="ligand shared between dimeric partners" FT Group: 1; Condition: C FT BINDING 76 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT Group: 2; Condition: C FT BINDING 77 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT /ligand_note="ligand shared between dimeric partners" FT Group: 1; Condition: C FT BINDING 79 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT Group: 2; Condition: C FT BINDING 84 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT Group: 2; Condition: C FT BINDING 94 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT Group: 2; Condition: C FT BINDING 250 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: H FT BINDING 278 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: C FT BINDING 323 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: C FT BINDING 417 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="3" FT Group: 4; Condition: C FT BINDING 420 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="3" FT Group: 4; Condition: C FT BINDING 423 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="3" FT Group: 4; Condition: C FT BINDING 427 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="4" FT Group: 5; Condition: C FT BINDING 455 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="4" FT Group: 5; Condition: C FT BINDING 458 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="4" FT Group: 5; Condition: C FT BINDING 461 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="4" FT Group: 5; Condition: C FT BINDING 465 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="3" FT Group: 4; Condition: C FT BINDING 523 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: C FT BINDING 552 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: C FT BINDING 587 FT /ligand="[Ni-4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:47739" FT Group: 3; Condition: C FT BINDING 117 FT /ligand="CO" FT /ligand_id="ChEBI:CHEBI:17245" FT Condition: H XX Size: 632-807; Related: None; Template: Q08368; Q46G04; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: in ARCFU, METAC, METKA, METMA, METTE Plasmid: None Comments: None XX # Revision 1.25 2023/06/01 //