AC   MF_01182;
DC   Protein; auto
TR   HAMAP; MF_01182; -; 1; level=0
XX
Names: Cytochrom_C552
XX
ID   NRFA
DE   RecName: Full=Cytochrome c-552;
DE            EC=1.7.2.2;
DE   AltName: Full=Ammonia-forming cytochrome c nitrite reductase;
DE            Short=Cytochrome c nitrite reductase;
DE   Flags: Precursor;
GN   Name=nrfA;
XX
CC   -!- FUNCTION: Catalyzes the reduction of nitrite to ammonia, consuming six
CC       electrons in the process.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=6 Fe(III)-[cytochrome c] + 2 H2O + NH4(+) = 6 Fe(II)-
CC         [cytochrome c] + 8 H(+) + nitrite; Xref=Rhea:RHEA:13089, Rhea:RHEA-
CC         COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.7.2.2;
case <FTGroup:2>
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC       Note=Binds 1 Ca(2+) ion per monomer.;
end case
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
CC   -!- COFACTOR:
CC       Name=heme c; Xref=ChEBI:CHEBI:61717;
CC       Note=Binds 5 heme c groups covalently per monomer.;
end case
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- SIMILARITY: Belongs to the cytochrome c-552 family.
XX
DR   PROSITE; PS51008; MULTIHEME_CYTC; 1; trigger=no
DR   Pfam; PF02335; Cytochrom_C552; 1; trigger=no
DR   General; Signal; -; 1; trigger=yes
XX
case <FTGroup:2>
KW   Calcium
end case
KW   Electron transport
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
KW   Heme
end case
case <FTGroup:1>
KW   Iron
KW   Metal-binding
end case
KW   Oxidoreductase
KW   Periplasm
KW   Signal
KW   Transport
XX
case <FTGroup:2>
GO   GO:0005509; F:calcium ion binding
end case
case <FTGroup:1>
GO   GO:0005506; F:iron ion binding
end case
GO   GO:0042279; F:nitrite reductase (cytochrome, ammonia-forming) activity
GO   GO:0006807; P:nitrogen compound metabolic process
GO   GO:0042597; C:periplasmic space
XX
FT   From: NRFA_ECOLI (P0ABK9)
FT   BINDING         94
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         126
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: K
FT   BINDING         164
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         213
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         215
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT   Group: 2; Condition: E
FT   BINDING         216
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT   Group: 2; Condition: Y
FT   BINDING         261
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT   Group: 2; Condition: K
FT   BINDING         263
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT   Group: 2; Condition: Q
FT   BINDING         275
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="5"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         286
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="4"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         301
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         318
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="5"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         393
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="4"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   Group: 1; Condition: H
FT   BINDING         122
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT   Group: 3; Condition: C
FT   BINDING         125
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT   Group: 3; Condition: C
FT   BINDING         160
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT   Group: 4; Condition: C
FT   BINDING         163
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT   Group: 4; Condition: C
FT   BINDING         209
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /note="covalent"
FT   Group: 5; Condition: C
FT   BINDING         212
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /note="covalent"
FT   Group: 5; Condition: C
FT   BINDING         216
FT                   /ligand="substrate"
FT   Condition: Y
FT   BINDING         264
FT                   /ligand="substrate"
FT   Condition: H
FT   BINDING         282
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="4"
FT                   /note="covalent"
FT   Group: 6; Condition: C
FT   BINDING         285
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="4"
FT                   /note="covalent"
FT   Group: 6; Condition: C
FT   BINDING         314
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="5"
FT                   /note="covalent"
FT   Group: 7; Condition: C
FT   BINDING         317
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="5"
FT                   /note="covalent"
FT   Group: 7; Condition: C
XX
Size: 463-538;
Related: None;
Template: P0ABK9;
Scope:
 Bacteria; Bacteroidota
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.32 2023/01/26
//