AC   MF_01209;
DC   Protein; auto
TR   HAMAP; MF_01209; -; 1; level=0
XX
Names: CPSase_S_chain
XX
ID   CARA
DE   RecName: Full=Carbamoyl phosphate synthase small chain;
DE            EC=6.3.5.5;
DE   AltName: Full=Carbamoyl phosphate synthetase glutamine chain;
GN   Name=carA;
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CC   -!- FUNCTION: Small subunit of the glutamine-dependent carbamoyl phosphate
CC       synthetase (CPSase). CPSase catalyzes the formation of carbamoyl
CC       phosphate from the ammonia moiety of glutamine, carbonate, and
CC       phosphate donated by ATP, constituting the first step of 2 biosynthetic
CC       pathways, one leading to arginine and/or urea and the other to
CC       pyrimidine nucleotides. The small subunit (glutamine amidotransferase)
CC       binds and cleaves glutamine to supply the large subunit with the
CC       substrate ammonia.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP +
CC         carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate;
CC         Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359,
CC         ChEBI:CHEBI:456216; EC=6.3.5.5;
CC   -!- CATALYTIC ACTIVITY: [<name>]:
CC       Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC         Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:58359;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl
CC       phosphate from bicarbonate: step 1/1.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       (S)-dihydroorotate from bicarbonate: step 1/3.
CC   -!- SUBUNIT: Composed of two chains; the small (or glutamine) chain
CC       promotes the hydrolysis of glutamine to ammonia, which is used by the
CC       large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of
CC       heterodimers (alpha,beta)4.
case <OG:Chloroplast>
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
end case
CC   -!- SIMILARITY: Belongs to the CarA family.
XX
DR   Pfam; PF00117; GATase; 1; trigger=no
DR   Pfam; PF00988; CPSase_sm_chain; 1; trigger=no
DR   PRINTS; PR00099; CPSGATASE; 1; trigger=no
DR   PRINTS; PR00096; GATASE; 1; trigger=no
DR   PROSITE; PS51273; GATASE_TYPE_1; 1; trigger=yes
DR   NCBIfam; TIGR01368; CPSaseIIsmall; 1; trigger=no
XX
KW   Amino-acid biosynthesis
KW   Arginine biosynthesis
KW   ATP-binding
KW   Glutamine amidotransferase
KW   Ligase
KW   Nucleotide-binding
KW   Pyrimidine biosynthesis
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GO   GO:0005524; F:ATP binding
GO   GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
GO   GO:0006526; P:arginine biosynthetic process
GO   GO:0006221; P:pyrimidine nucleotide biosynthetic process
case <OG:Chloroplast>
GO   GO:0009507; C:chloroplast
end case
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FT   From: CARA_ECOLI (P0A6F1)
FT   REGION          Nter..189
FT                   /note="CPSase"
FT   ACT_SITE        269
FT                   /note="Nucleophile"
FT   Condition: C
FT   ACT_SITE        353
FT   Condition: H
FT   ACT_SITE        355
FT   Condition: [ED]
FT   BINDING         47
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: S
FT   BINDING         241
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: G
FT   BINDING         243
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: G
FT   BINDING         270
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: [LFM]
FT   BINDING         273
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: Q
FT   BINDING         311
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: N
FT   BINDING         313
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: G
FT   BINDING         314
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Condition: [YF]
XX
Size: 345-407;
Related: None;
Template: P0A6F1; P54324; Q9L4N5; P77885; O08317;
Scope:
 Bacteria
 Archaea
 Plastid
Fusion:
 Nter: None
 Cter: None
Duplicate: in HALH5, BACSU, LACPL
Plasmid: None
Comments: Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of
 this family: one specific for arginine biosynthesis (called CARX_ in ID, and
 CARA in GN) and the other specific for pyrimidine synthesis (called CARA_ in
 ID, and PYRAA in GN).
 Weird insert in THEMA; not shown in alignment.
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# Revision 1.48 2023/09/19
//