AC   MF_01225;
DC   Protein; auto
c?   <OC:Bacteria>
TR   HAMAP; MF_01225_B; -; 1; level=0
c?   <OC:Archaea>
TR   HAMAP; MF_01225_A; -; 1; level=0
XX
Names: MoaA
XX
ID   MOAA
case <OC:Bacteria>
DE   RecName: Full=GTP 3',8-cyclase;
DE            EC=4.1.99.22;
DE   AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
case <OC:Archaea>
DE   RecName: Full=Probable GTP 3',8-cyclase;
DE            EC=4.1.99.22;
DE   AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
GN   Name=moaA;
XX
CC   -!- FUNCTION: Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-
CC       dihydroguanosine 5'-triphosphate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-
CC         dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-
CC         methionine; Xref=Rhea:RHEA:49576, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:37565, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:131766; EC=4.1.99.22;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated
CC       with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1
CC       [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived
CC       substrate.;
CC   -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
case <OC:Bacteria>
CC   -!- SUBUNIT: Monomer and homodimer.
end case
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. MoaA family.
XX
DR   PROSITE; PS01305; MOAA_NIFB_PQQE; 1; trigger=no
DR   Pfam; PF06463; Mob_synth_C; 1; trigger=no
DR   Pfam; PF04055; Radical_SAM; 1; trigger=no
DR   NCBIfam; TIGR02666; MoaA; 1; trigger=no
DR   NCBIfam; TIGR02668; moaA_archaeal; 1; trigger=no
XX
KW   4Fe-4S
KW   GTP-binding
KW   Iron
KW   Iron-sulfur
KW   Lyase
KW   Metal-binding
KW   Molybdenum cofactor biosynthesis
KW   Nucleotide-binding
KW   S-adenosyl-L-methionine
XX
GO   GO:0005525; F:GTP binding
GO   GO:0051539; F:4 iron, 4 sulfur cluster binding
GO   GO:0061798; F:GTP 3',8'-cyclase activity
GO   GO:1904047; F:S-adenosyl-L-methionine binding
GO   GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process
XX
FT   From: MOAA_STAA8 (P69848)
case <OC:Bacteria>
FT   BINDING         266..268
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [RK]-x-R
FT   BINDING         24
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         28
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         31
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         261
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         264
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         278
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         17
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: R
FT   BINDING         30
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: Y
FT   BINDING         71
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: R
FT   BINDING         75
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G
FT   BINDING         102
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [TS]
FT   BINDING         126
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: S
FT   BINDING         163
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: K
FT   BINDING         197
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: M
end case
FT   From: MOAA_PYRAB (Q9V2G2)
case <OC:Archaea>
FT   BINDING         255..257
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: R-x-R
FT   BINDING         21
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         25
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         28
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Condition: C
FT   BINDING         250
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         253
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         267
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-substrate"
FT   Condition: C
FT   BINDING         14
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: R
FT   BINDING         27
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: Y
FT   BINDING         61
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [KR]
FT   BINDING         65
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G
FT   BINDING         90
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: T
FT   BINDING         114
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: S
FT   BINDING         150
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: K
FT   BINDING         189
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: M
end case
XX
case <OC:Bacteria>
Size: 318-378;
end case
case <OC:Archaea>
Size: 296-357;
end case
Related: None;
Template: P69848; Q44118; P30745; Q9X5W3;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: in MYCTU, PSEAE
Plasmid: in ARTNI
Comments: None
XX
# Revision 1.35 2023/06/01
//