AC MF_01253; DC Protein; auto TR HAMAP; MF_01253; -; 1; level=0 XX Names: Endonuclease_8 XX ID END8 DE RecName: Full=Endonuclease 8; DE AltName: Full=DNA glycosylase/AP lyase Nei; DE EC=3.2.2.-; DE EC=4.2.99.18; DE AltName: Full=DNA-(apurinic or apyrimidinic site) lyase Nei; DE AltName: Full=Endonuclease VIII; GN Name=nei; XX CC -!- FUNCTION: Involved in base excision repair of DNA damaged by oxidation CC or by mutagenic agents. Acts as DNA glycosylase that recognizes and CC removes damaged bases. Has a preference for oxidized pyrimidines, such CC as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP CC (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA CC strand. Cleaves the DNA backbone by beta-delta elimination to generate CC a single-strand break at the site of the removed base with both 3'- and CC 5'-phosphates. CC -!- CATALYTIC ACTIVITY: CC Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- CC deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- CC dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- CC deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- CC COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, CC ChEBI:CHEBI:167181; EC=4.2.99.18; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Note=Binds 1 zinc ion per subunit.; CC -!- SIMILARITY: Belongs to the FPG family. XX DR Pfam; PF01149; Fapy_DNA_glyco; 1; trigger=no DR Pfam; PF06831; H2TH; 1; trigger=no DR Pfam; PF06827; zf-FPG_IleRS; 1; trigger=no DR PROSITE; PS51068; FPG_CAT; 1; trigger=no DR PROSITE; PS01242; ZF_FPG_1; 1; trigger=no DR PROSITE; PS51066; ZF_FPG_2; 1; trigger=yes XX KW DNA damage KW DNA repair KW DNA-binding KW Glycosidase KW Hydrolase KW Lyase KW Multifunctional enzyme case KW Metal-binding KW Zinc KW Zinc-finger end case XX GO GO:0003906; F:DNA-(apurinic or apyrimidinic site) endonuclease activity GO GO:0000703; F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity case GO GO:0008270; F:zinc ion binding end case GO GO:0006281; P:DNA repair XX FT From: END8_ECOLI (P50465) FT INIT_MET Nter FT /note="Removed" FT Condition: M FT ACT_SITE 2 FT /note="Schiff-base intermediate with DNA" FT Condition: P FT ACT_SITE 3 FT /note="Proton donor" FT Condition: E FT ACT_SITE 53 FT /note="Proton donor; for beta-elimination activity" FT Condition: K FT ACT_SITE 253 FT /note="Proton donor; for delta-elimination activity" FT Condition: R FT BINDING 70 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Condition: Q FT BINDING 125 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Condition: R FT BINDING 169 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Condition: N XX Size: 260-265; Related: None; Template: P50465; Scope: Bacteria; Enterobacterales Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.31 2024/01/30 //