AC   MF_01289;
DC   Protein; auto
TR   HAMAP; MF_01289; -; 1; level=0
XX
Names: Galacton_dehydrat
XX
ID   DGOD
DE   RecName: Full=D-galactonate dehydratase;
DE            Short=GalD;
DE            EC=4.2.1.6;
GN   Name=dgoD;
XX
CC   -!- FUNCTION: Catalyzes the dehydration of D-galactonate to 2-keto-3-deoxy-
CC       D-galactonate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O;
CC         Xref=Rhea:RHEA:18649, ChEBI:CHEBI:12931, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:57989; EC=4.2.1.6;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 1 Mg(2+) ion per subunit.;
end case
CC   -!- PATHWAY: Carbohydrate acid metabolism; D-galactonate degradation; D-
CC       glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 1/3.
CC   -!- MISCELLANEOUS: Reaction proceeds via an anti dehydration.
CC   -!- SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing
CC       enzyme family. GalD subfamily.
XX
DR   PROSITE; PS00908; MR_MLE_1; 1; trigger=no
DR   PROSITE; PS00909; MR_MLE_2; 1; trigger=no
DR   Pfam; PF01188; MR_MLE; 1; trigger=no
DR   Pfam; PF02746; MR_MLE_N; 1; trigger=no
XX
KW   Lyase
case <FTGroup:1>
KW   Magnesium
KW   Metal-binding
end case
XX
case <FTGroup:1>
GO   GO:0000287; F:magnesium ion binding
end case
GO   GO:0008869; F:galactonate dehydratase activity
GO   GO:0034194; P:D-galactonate catabolic process
XX
FT   From: DGOD_ECOLI (Q6BF17)
FT   ACT_SITE        185
FT                   /note="Proton donor/acceptor"
FT   Condition: H
FT   ACT_SITE        285
FT                   /note="Proton donor/acceptor"
FT   Condition: H
FT   BINDING         183
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: D
FT   BINDING         209
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: E
FT   BINDING         235
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: E
FT   SITE            258
FT                   /note="Increases basicity of active site His"
FT   Condition: D
FT   SITE            310
FT                   /note="Transition state stabilizer"
FT   Condition: E
XX
Size: 365-385;
Related: None;
Template: Q6BF17;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: in ECOSM
Plasmid: None
Comments: None
XX
# Revision 1.17 2023/01/26
//