AC MF_01297; DC Protein; auto TR HAMAP; MF_01297; -; 1; level=0 XX Names: nitrobindin XX case ID NB DE RecName: Full=Peroxynitrite isomerase; DE EC=5.99.-.-; DE AltName: Full=Ferric nitrobindin; DE Short=Nb(III); else DE RecName: Full=Ferric nitrobindin-like protein; end case XX case CC -!- FUNCTION: Heme-binding protein able to scavenge peroxynitrite and to CC protect free L-tyrosine against peroxynitrite-mediated nitration, by CC acting as a peroxynitrite isomerase that converts peroxynitrite to CC nitrate. Therefore, this protein likely plays a role in peroxynitrite CC sensing and in the detoxification of reactive nitrogen and oxygen CC species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) CC in vitro, but may act as a sensor of peroxynitrite levels in vivo. CC -!- CATALYTIC ACTIVITY: CC Reaction=peroxynitrite = nitrate; Xref=Rhea:RHEA:63116, CC ChEBI:CHEBI:17632, ChEBI:CHEBI:25941; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63117; CC -!- COFACTOR: CC Name=heme b; Xref=ChEBI:CHEBI:60344; CC Note=Binds 1 heme b group per subunit, that coordinates a highly CC solvent-exposed Fe(III) atom. CC -!- PATHWAY: Nitrogen metabolism. CC -!- DOMAIN: Forms a 10-stranded antiparallel beta-barrel structure able to CC accommodate a hydrophobic ligand in its interior. In fact, this fold CC hosts the heme group, which is located in a wide surface cleft. else CC -!- CAUTION: Lacks the conserved His residue that binds heme iron in the CC nitrobindin family. end case CC -!- SIMILARITY: Belongs to the nitrobindin family. XX DR Pfam; PF08768; DUF1794; 1; trigger=no XX case KW Heme KW Iron KW Metal-binding KW Isomerase end case XX case GO GO:0020037; F:heme binding GO GO:0062213; F:peroxynitrite isomerase activity end case XX FT From: NB1_MYCTU (P9WFG7) FT MOTIF 17..23 FT /note="GXWXGXG" FT Condition: G-x-W-x-G-x-G FT BINDING 155 FT /ligand="heme b" FT /ligand_id="ChEBI:CHEBI:60344" FT /ligand_part="Fe" FT /ligand_part_id="ChEBI:CHEBI:18248" FT /note="axial binding residue" FT Tag: heme_iron_binding; Condition: H FT BINDING 29 FT /ligand="heme b" FT /ligand_id="ChEBI:CHEBI:60344" FT Optional; Condition: T FT BINDING 123 FT /ligand="heme b" FT /ligand_id="ChEBI:CHEBI:60344" FT Optional; Condition: K XX Size: 147-248; Related: None; Template: P9WFG9; P9WFG7; Scope: Bacteria; Actinomycetota Fusion: Nter: None Cter: None Duplicate: in MYCA1, MYCBO, MYCBP, MYCGI, MYCLE, MYCMM, MYCPA, MYCS2, MYCSJ, MYCSS, MYCTA, MYCTU, MYCUA, MYCVP, NOCFA, RHOJR, STRAW, STRGG Plasmid: None Comments: None XX # Revision 1.15 2023/01/13 //