AC   MF_01324;
DC   Protein; auto
c?   <OC:Cyanobacteriota> or <OG:Chloroplast>
TR   HAMAP; MF_01324; -; 1; level=0
XX
Names: RNApol_bact_RpoC2
XX
ID   RPOC2
case <OC:Cyanobacteriota>
DE   RecName: Full=DNA-directed RNA polymerase subunit beta';
DE            Short=RNAP subunit beta';
DE            EC=2.7.7.6;
DE   AltName: Full=RNA polymerase subunit beta';
DE   AltName: Full=Transcriptase subunit beta';
else case <OG:Chloroplast>
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'';
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'';
DE            Short=RNA polymerase subunit beta'';
end case
GN   Name=rpoC2;
XX
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 1 Zn(2+) ion per subunit.;
end case
case <OC:Cyanobacteriota>
CC   -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC       alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC       factor is associated with the core the holoenzyme is formed, which can
CC       initiate transcription.
else case <OG:Chloroplast>
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
end case
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily.
XX
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 0-1; trigger=no
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1; trigger=no
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1-2; trigger=no
DR   NCBIfam; TIGR02388; rpoC2_cyan; 0-2; trigger=no
XX
case <FTGroup:1>
KW   Metal-binding
KW   Zinc
end case
KW   Transcription
KW   Transferase
KW   Nucleotidyltransferase
KW   DNA-directed RNA polymerase
XX
GO   GO:0003677; F:DNA binding
GO   GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity
GO   GO:0006351; P:DNA-templated transcription
case <FTGroup:1>
GO   GO:0008270; F:zinc ion binding
end case
case <OG:Chloroplast>
GO   GO:0009507; C:chloroplast
end case
XX
FT   From: RPOC2_NOSS1 (P22705)
FT   BINDING         219
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         293
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         300
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         303
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
XX
Size: 1011-1595;
Related: MF_01322!;
Template: P22705;
Scope:
 Bacteria; Cyanobacteriota
 Plastid
Fusion:
 Nter: MF_01323 (rpoC1)
 Cter: None
Duplicate: None
Plasmid: None
Comments: In cyanobacteria and plastids, there are two ORFs (rpoC1, MF_01323 and
 this one, rpoC2, MF_01324) that together are equivalent to the beta'
 subunit found in all other bacteria (rpoC, MF_01322).
 However,in the red algae CYAM1 the rpoC1 and rpoC2 genes are fused into
 one ORF. This seems to be the result of evolution acting to reduce protein
 size rather than horizontal transfer from a bacteria.
 Some algal proteins with inserts are not included in the alignment. ASTLO
 and EUGGR have large deletions and are not included in the alignments.
XX
# Revision 1.38 2023/06/01
//