AC   MF_01327;
DC   Protein; auto
TR   HAMAP; MF_01327; -; 1; level=0
XX
Names: TMP_synthase_cyanobact
XX
ID   THIE
DE   RecName: Full=Thiamine-phosphate synthase;
DE            Short=TP synthase;
DE            Short=TPS;
DE            EC=2.5.1.3;
DE   AltName: Full=Thiamine-phosphate pyrophosphorylase;
DE            Short=TMP pyrophosphorylase;
DE            Short=TMP-PPase;
GN   Name=thiE;
XX
CC   -!- FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole
CC       monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine
CC       pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl
CC         phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2
CC         H(+) = CO2 + diphosphate + thiamine phosphate; Xref=Rhea:RHEA:47844,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62899; EC=2.5.1.3;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-
CC         methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate
CC         + thiamine phosphate; Xref=Rhea:RHEA:47848, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575,
CC         ChEBI:CHEBI:57841, ChEBI:CHEBI:62890; EC=2.5.1.3;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-
CC         5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine
CC         phosphate; Xref=Rhea:RHEA:22328, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841,
CC         ChEBI:CHEBI:58296; EC=2.5.1.3;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 1 Mg(2+) ion per subunit.;
end case
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis;
CC       thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine
CC       and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the thiamine-phosphate synthase family.
XX
DR   Pfam; PF02581; TMP-TENI; 1; trigger=no
DR   NCBIfam; TIGR00693; ThiE; 1; trigger=no
DR   PIRSF; PIRSF000512; TMP_PPase_Cyanobac_prd; 1; trigger=no
XX
KW   Thiamine biosynthesis
KW   Transferase
case <FTGroup:1>
KW   Magnesium
KW   Metal-binding
end case
XX
GO   GO:0000287; F:magnesium ion binding
GO   GO:0004789; F:thiamine-phosphate diphosphorylase activity
GO   GO:0009228; P:thiamine biosynthetic process
XX
FT   From: THIE_NOSS1 (Q8YX72)
FT   REGION          Nter..129
FT                   /note="Unknown"
FT   REGION          130..Cter
FT                   /note="Thiamine-phosphate synthase"
FT   BINDING         177..181
FT                   /ligand="4-amino-2-methyl-5-
FT                   (diphosphooxymethyl)pyrimidine"
FT                   /ligand_id="ChEBI:CHEBI:57841"
FT   Condition: Q-x-R-x-K
FT   BINDING         274..276
FT                   /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-
FT                   ylidene]ethyl phosphate"
FT                   /ligand_id="ChEBI:CHEBI:62899"
FT   Optional; Condition: T-x-T
FT   BINDING         210
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: [DN]
FT   BINDING         229
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: [DE]
FT   BINDING         209
FT                   /ligand="4-amino-2-methyl-5-
FT                   (diphosphooxymethyl)pyrimidine"
FT                   /ligand_id="ChEBI:CHEBI:57841"
FT   Condition: N
FT   BINDING         248
FT                   /ligand="4-amino-2-methyl-5-
FT                   (diphosphooxymethyl)pyrimidine"
FT                   /ligand_id="ChEBI:CHEBI:57841"
FT   Condition: S
FT   BINDING         277
FT                   /ligand="4-amino-2-methyl-5-
FT                   (diphosphooxymethyl)pyrimidine"
FT                   /ligand_id="ChEBI:CHEBI:57841"
FT   Condition: K
FT   BINDING         304
FT                   /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-
FT                   ylidene]ethyl phosphate"
FT                   /ligand_id="ChEBI:CHEBI:62899"
FT   Condition: G
XX
Size: 341-353;
Related: MF_00097;
Template: Q8YX72;
Scope:
 Bacteria; Cyanobacteriota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: Unknown N-terminal domain in Cyanobacteriota. All other bacteria without
 this domain belong to family MF_00097.
XX
# Revision 1.33 2023/06/01
//