AC MF_01349; DC Protein; auto TR HAMAP; MF_01349; -; 1; level=0 XX Names: PanCY XX ID PANCY DE RecName: Full=Bifunctional pantoate ligase/cytidylate kinase; DE Includes: DE RecName: Full=Pantothenate synthetase; DE Short=PS; DE EC=6.3.2.1; DE AltName: Full=Pantoate--beta-alanine ligase; DE AltName: Full=Pantoate-activating enzyme; DE Includes: DE RecName: Full=Cytidylate kinase; DE Short=CK; DE EC=2.7.4.25; DE AltName: Full=Cytidine monophosphate kinase; DE Short=CMP kinase; GN Name=panC/cmk; XX CC -!- FUNCTION: Catalyzes the condensation of pantoate with beta-alanine in CC an ATP-dependent reaction via a pantoyl-adenylate intermediate. CC -!- FUNCTION: Catalyzes the transfer of a phosphate group from ATP to CC either CMP or dCMP to form CDP or dCDP and ADP, respectively. CC -!- CATALYTIC ACTIVITY: CC Reaction=(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + CC diphosphate + H(+); Xref=Rhea:RHEA:10912, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15980, ChEBI:CHEBI:29032, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57966, ChEBI:CHEBI:456215; EC=6.3.2.1; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + CMP = ADP + CDP; Xref=Rhea:RHEA:11600, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58069, ChEBI:CHEBI:60377, CC ChEBI:CHEBI:456216; EC=2.7.4.25; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + dCMP = ADP + dCDP; Xref=Rhea:RHEA:25094, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:57566, ChEBI:CHEBI:58593, CC ChEBI:CHEBI:456216; EC=2.7.4.25; CC -!- PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)- CC pantothenate from (R)-pantoate and beta-alanine: step 1/1. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: In the N-terminal section; belongs to the pantothenate CC synthetase family. CC -!- SIMILARITY: In the C-terminal section; belongs to the cytidylate kinase CC family. Type 1 subfamily. XX DR Pfam; PF02224; Cytidylate_kin; 1; trigger=no DR Pfam; PF02569; Pantoate_ligase; 1; trigger=no DR NCBIfam; TIGR00017; Cmk; 1; trigger=no DR NCBIfam; TIGR00018; PanC; 1; trigger=no DR NCBIfam; TIGR00125; Cyt_tran_rel; 1; trigger=no XX KW Cytoplasm KW ATP-binding KW Kinase KW Ligase KW Multifunctional enzyme KW Nucleotide-binding KW Pantothenate biosynthesis KW Transferase XX GO GO:0004592; F:pantoate-beta-alanine ligase activity GO GO:0005524; F:ATP binding GO GO:0004127; F:cytidylate kinase activity GO GO:0006220; P:pyrimidine nucleotide metabolic process GO GO:0015940; P:pantothenate biosynthetic process GO GO:0005737; C:cytoplasm XX FT From: PANCY_SYNY3 (Q55074) FT BINDING 30..37 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: M-x(3)-H-x(2)-H FT BINDING 150..153 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-x(2)-D FT BINDING 187..190 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: x(3)-R FT REGION Nter..283 FT /note="Pantoate--beta-alanine ligase" FT REGION 284..Cter FT /note="Cytidylate kinase" FT ACT_SITE 37 FT /note="Proton donor" FT Condition: H FT BINDING 61 FT /ligand="beta-alanine" FT /ligand_id="ChEBI:CHEBI:57966" FT Condition: Q FT BINDING 61 FT /ligand="(R)-pantoate" FT /ligand_id="ChEBI:CHEBI:15980" FT Condition: Q FT BINDING 156 FT /ligand="(R)-pantoate" FT /ligand_id="ChEBI:CHEBI:15980" FT Condition: Q FT BINDING 179 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Optional; Condition: [VLIGA] XX Size: 480-542; Related: MF_00158; MF_00238; MF_00239; Template: P9WIL5; P0A6I0; Scope: Bacteria; Cyanobacteriota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: This fusion protein seems only to exist in Cyanobacteriota; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity. XX # Revision 1.22 2023/06/01 //