AC MF_01428; DC Protein; auto TR HAMAP; MF_01428; -; 1; level=0 XX Names: Glu_Q_tRNA_synth XX ID GLUQ DE RecName: Full=Glutamyl-Q tRNA(Asp) synthetase; DE Short=Glu-Q-RSs; DE EC=6.1.1.-; GN Name=gluQ; XX CC -!- FUNCTION: Catalyzes the tRNA-independent activation of glutamate in CC presence of ATP and the subsequent transfer of glutamate onto a CC tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- CC cyclopenten-1-yl) moiety of the queuosine in the wobble position of the CC QUC anticodon. case CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Note=Binds 1 zinc ion per subunit.; end case CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. CC GluQ subfamily. XX DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1; trigger=no DR Pfam; PF00749; tRNA-synt_1c; 1; trigger=no DR PRINTS; PR00987; TRNASYNTHGLU; 1; trigger=no DR NCBIfam; TIGR03838; queuosine_YadB; 1; trigger=no XX KW Aminoacyl-tRNA synthetase KW ATP-binding KW Ligase KW Nucleotide-binding case KW Metal-binding KW Zinc end case XX GO GO:0004812; F:aminoacyl-tRNA ligase activity case GO GO:0008270; F:zinc ion binding end case XX FT From: GLUQ_ECOLI (P27305) FT BINDING 19..23 FT /ligand="L-glutamate" FT /ligand_id="ChEBI:CHEBI:29985" FT Optional; Condition: R-[FLY]-[AS]-P-[ST] FT MOTIF 22..32 FT /note="'HIGH' region" FT MOTIF 238..242 FT /note="'KMSKS' region" FT BINDING 111 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Group: 1; Condition: C FT BINDING 113 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Group: 1; Condition: C FT BINDING 125 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Group: 1; Condition: Y FT BINDING 129 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Group: 1; Condition: C FT BINDING 55 FT /ligand="L-glutamate" FT /ligand_id="ChEBI:CHEBI:29985" FT Optional; Condition: [ED] FT BINDING 182 FT /ligand="L-glutamate" FT /ligand_id="ChEBI:CHEBI:29985" FT Optional; Condition: Y FT BINDING 200 FT /ligand="L-glutamate" FT /ligand_id="ChEBI:CHEBI:29985" FT Optional; Condition: R FT BINDING 241 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: K XX Size: 283-347; Related: MF_00022!; Template: P27305; Scope: Bacteria Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: See: PubMed=16164993; Blaise M., Becker H.D., Lapointe J., Cambillau C., Giege R., Kern D.; "Glu-Q-tRNA(Asp) synthetase coded by the yadB gene, a new paralog of aminoacyl-tRNA synthetase that glutamylates tRNA(Asp) anticodon."; Biochimie 87:847-861(2005). XX # Revision 1.22 2023/06/01 //