AC MF_01438; DC Protein; auto TR HAMAP; MF_01438; -; 1; level=0 XX Names: DisA XX ID DISA DE RecName: Full=DNA integrity scanning protein DisA; DE AltName: Full=Diadenylate cyclase; DE EC=2.7.7.85; DE AltName: Full=Cyclic di-AMP synthase; DE Short=c-di-AMP synthase; GN Name=disA; XX case or or or CC -!- FUNCTION: Participates in a DNA-damage check-point that is active prior CC to asymmetric division when DNA is damaged. DisA forms globular foci CC that rapidly scan along the chromosomes during sporulation, searching CC for lesions. When a lesion is present, DisA pauses at the lesion site. CC This triggers a cellular response that culminates in a temporary block CC in sporulation initiation. CC -!- FUNCTION: Has also diadenylate cyclase activity, catalyzing the CC condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP CC acts as a signaling molecule that couples DNA integrity with CC progression of sporulation. The rise in c-di-AMP level generated by CC DisA while scanning the chromosome, operates as a positive signal that CC advances sporulation; upon encountering a lesion, the DisA focus CC arrests at the damaged site and halts c-di-AMP synthesis. else CC -!- FUNCTION: Participates in a DNA-damage check-point. DisA forms globular CC foci that rapidly scan along the chromosomes searching for lesions. CC -!- FUNCTION: Has also diadenylate cyclase activity, catalyzing the CC condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP CC likely acts as a signaling molecule that may couple DNA integrity with CC a cellular process. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC -!- SUBUNIT: Homooctamer. CC -!- SIMILARITY: Belongs to the DisA family. XX DR PROSITE; PS51794; DAC; 1; trigger=yes DR Pfam; PF10635; DisA-linker; 1; trigger=no DR Pfam; PF02457; DisA_N; 1; trigger=no DR Pfam; PF00633; HHH; 1; trigger=no XX KW ATP-binding KW DNA damage KW DNA repair KW DNA-binding KW Nucleotide-binding KW Nucleotidyltransferase KW Magnesium KW Transferase XX GO GO:0003677; F:DNA binding GO GO:0005524; F:ATP binding GO GO:0016779; F:nucleotidyltransferase activity GO GO:0006281; P:DNA repair GO GO:0019932; P:second-messenger-mediated signaling XX FT From: DISA_THEMA (Q9WY43) FT BINDING 107..111 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: x-R-x-x-[TS] FT BINDING 76 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G FT BINDING 94 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [LV] XX Size: 344-374; Related: MF_00838; MF_00840; MF_01499; Template: P37573; Q9WY43; P9WNW5; A0R564; Q9X8L6; A0R8F5; Q6HPT4; Scope: Bacteria; Actinomycetota Bacteria; Bacillaceae Bacteria; Clostridia Bacteria; Fusobacterium Bacteria; Thermotoga Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.20 2023/01/13 //