AC   MF_01472;
DC   Protein; auto
TR   HAMAP; MF_01472; -; 1; level=0
XX
Names: GtfA
XX
ID   GTFA
DE   RecName: Full=UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit;
DE            EC=2.4.1.-;
DE   AltName: Full=Glycosyltransferase GtfA;
GN   Name=gtfA;
XX
CC   -!- FUNCTION: Required for polymorphic O-glycosylation of the serine-rich
CC       repeat protein in this bacteria. Catalyzes the first step in
CC       glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to
CC       serine residues in the substrate protein. Part of the accessory
CC       SecA2/SecY2 system specifically required to export serine-rich repeat
CC       cell wall proteins usually encoded upstream in the same operon.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-
CC         acetyl-alpha-D-glucosaminyl]-L-seryl-[protein] + H(+) + UDP;
CC         Xref=Rhea:RHEA:59872, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:15471,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57705,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:143279;
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- SUBUNIT: Forms a heterotetramer with 2 subunits each of GtfA and GtfB.
CC       Part of the accessory SecA2/SecY2 protein translocation apparatus.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane
CC       protein. Note=Cell membrane association requires GtfB.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily.
XX
DR   Pfam; PF00534; Glycos_transf_1; 1; trigger=no
DR   NCBIfam; TIGR02918; TIGR02918; 1; trigger=no
XX
KW   Cell membrane
KW   Cytoplasm
KW   Glycosyltransferase
KW   Membrane
KW   Nucleotide-binding
KW   Transferase
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GO   GO:0018242; P:protein O-linked glycosylation via serine
GO   GO:0016757; F:glycosyltransferase activity
GO   GO:0017122; C:protein N-acetylglucosaminyltransferase complex
GO   GO:0005886; C:plasma membrane
GO   GO:0005737; C:cytoplasm
XX
FT   From: GTFA_STRPN (A0A0H2URG7)
FT   BINDING         16..19
FT                   /ligand="UDP"
FT                   /ligand_id="ChEBI:CHEBI:58223"
FT   Condition: G-V-E-Y
FT   BINDING         384..385
FT                   /ligand="UDP"
FT                   /ligand_id="ChEBI:CHEBI:58223"
FT   Condition: [HQ]-[AHKQRVY]
FT   BINDING         404..407
FT                   /ligand="N-acetyl-D-glucosamine"
FT                   /ligand_id="ChEBI:CHEBI:506227"
FT   Condition: E-G-F-G
FT   BINDING         242
FT                   /ligand="N-acetyl-D-glucosamine"
FT                   /ligand_id="ChEBI:CHEBI:506227"
FT   Condition: H
XX
Size: 490-548;
Related: None;
Template: A0A0H2URG7; A0A0S4NM89; A1C3L9; Q3S2Y2; Q9AET5;
Scope:
 Bacteria; Bacillota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.15 2023/06/01
//