AC   MF_01490;
DC   Protein; auto
TR   HAMAP; MF_01490; -; 1; level=0
XX
Names: HJ_Resolv_Hjc
XX
ID   HJC
DE   RecName: Full=Crossover junction endodeoxyribonuclease Hjc;
DE            Short=Hjc;
DE            EC=3.1.21.10;
DE   AltName: Full=Holliday junction resolvase Hjc;
GN   Name=hjc;
XX
CC   -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC       junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC       DNA junctions introducing paired nicks in opposing strands, leaving a
CC       5'-terminal phosphate and a 3'-terminal hydroxyl group that are
CC       subsequently ligated to produce recombinant products.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 1 Mg(2+) ion per subunit.;
end case
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
XX
DR   Pfam; PF01870; Hjc; 1; trigger=no
DR   PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1; trigger=no
XX
KW   DNA damage
KW   DNA recombination
KW   DNA repair
KW   DNA-binding
KW   Endonuclease
KW   Hydrolase
case <FTGroup:1>
KW   Metal-binding
KW   Magnesium
end case
KW   Nuclease
XX
GO   GO:0008821; F:crossover junction DNA endonuclease activity
case <FTGroup:1>
GO   GO:0000287; F:magnesium ion binding
end case
GO   GO:0006310; P:DNA recombination
GO   GO:0006281; P:DNA repair
XX
FT   From: HJC_SACS2 (Q7LXU0)
FT   ACT_SITE        32
FT   Condition: S
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: E
FT   BINDING         42
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: D
FT   BINDING         55
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: E
FT   SITE            57
FT                   /note="Transition state stabilizer"
FT   Condition: K
XX
Size: 108-183;
Related: None;
Template: Q7LXU0; O27336; F9VND5; E7FHX4;
Scope:
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: A second, related Holliday juntion resolving enzyme, Hje, with
 different characteristics exists in some Sulfolobaceae. Another may exist
 in Pyrococcus.
XX
# Revision 1.13 2023/08/11
//