AC   MF_01539;
DC   Protein; auto
TR   HAMAP; MF_01539; -; 1; level=0
XX
Names: TmcAL
XX
ID   TMCAL
DE   RecName: Full=tRNA(Met) cytidine acetate ligase;
DE            EC=6.3.4.-;
GN   Name=tmcAL;
XX
CC   -!- FUNCTION: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at
CC       the wobble position of elongator tRNA(Met), using acetate and ATP as
CC       substrates. First activates an acetate ion to form acetyladenylate (Ac-
CC       AMP) and then transfers the acetyl group to tRNA to form ac(4)C34.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetate + ATP + cytidine(34) in elongator tRNA(Met) = AMP +
CC         diphosphate + N(4)-acetylcytidine(34) in elongator tRNA(Met);
CC         Xref=Rhea:RHEA:58144, Rhea:RHEA-COMP:10693, Rhea:RHEA-COMP:10694,
CC         ChEBI:CHEBI:30089, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456215;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the TmcAL family.
XX
DR   Pfam; PF05636; HIGH_NTase1; 1; trigger=no
DR   NCBIfam; TIGR00125; cyt_tran_rel; 1; trigger=no
XX
KW   ATP-binding
KW   Cytoplasm
KW   Ligase
KW   Nucleotide-binding
KW   RNA-binding
KW   tRNA processing
KW   tRNA-binding
XX
GO   GO:0016879; F:ligase activity, forming carbon-nitrogen bonds
GO   GO:0006400; P:tRNA modification
GO   GO:0005737; C:cytoplasm
XX
FT   From: TMCAL_BACSU (O34513)
FT   BINDING         7..20
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   BINDING         187..188
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: R-I
FT   BINDING         101
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: G
FT   BINDING         162
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: N
case not <FT:2>
FT   BINDING         187
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: R
end case
XX
Size: 298-426;
Related: None;
Template: O34513;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.18 2023/06/01
//