AC   MF_01552;
DC   Protein; auto
TR   HAMAP; MF_01552; -; 1; level=0
XX
Names: RimK
XX
ID   RIMK
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
DE   RecName: Full=Ribosomal protein bS6--L-glutamate ligase;
DE            EC=6.3.2.-;
DE   AltName: Full=Poly-alpha-glutamate synthase;
DE   AltName: Full=Ribosomal protein bS6 modification protein;
else
DE   RecName: Full=Probable alpha-L-glutamate ligase;
DE            EC=6.3.2.-;
end case
GN   Name=rimK;
XX
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
CC   -!- FUNCTION: An L-glutamate ligase that catalyzes the ATP-dependent post-
CC       translational addition of glutamate residues to the C-terminus of
CC       ribosomal protein bS6 (RpsF). Is also able to catalyze the synthesis of
CC       poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected
CC       glutamate as substrate. The number of glutamate residues added to
CC       either RpsF or to poly-alpha-glutamate changes with pH.
end case
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=Binds 2 magnesium or manganese ions per subunit.;
end case
CC   -!- SIMILARITY: Belongs to the RimK family.
XX
DR   PROSITE; PS50975; ATP_GRASP; 1; trigger=yes
DR   Pfam; PF02222; ATP-grasp; 1; trigger=no
DR   NCBIfam; TIGR00768; RimK_fam; 1; trigger=no
XX
KW   ATP-binding
KW   Ligase
KW   Nucleotide-binding
KW   Protein biosynthesis
case <FTGroup:1>
KW   Magnesium
KW   Manganese
KW   Metal-binding
end case
XX
GO   GO:0005524; F:ATP binding
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
GO   GO:0018169; F:ribosomal S6-glutamic acid ligase activity
GO   GO:0018410; P:C-terminal protein amino acid modification
else
GO   GO:0016881; F:acid-amino acid ligase activity
end case
XX
FT   From: RIMK_ECOLI (P0C0U4)
FT   BINDING         178..179
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: [EDQ]-[YF]
FT   BINDING         211..213
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: R-[SA]-N
FT   BINDING         248
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Group: 1; Condition: D
FT   BINDING         248
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT   Group: 1; Condition: D
FT   BINDING         260
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Group: 1; Condition: E
FT   BINDING         260
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   Group: 1; Condition: E
FT   BINDING         260
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT   Group: 1; Condition: E
FT   BINDING         260
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT   Group: 1; Condition: E
FT   BINDING         262
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   Group: 1; Condition: N
FT   BINDING         262
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT   Group: 1; Condition: N
FT   BINDING         141
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: K
FT   BINDING         187
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: D
XX
Size: 291-313;
Related: None;
Template: P0C0U4;
Scope:
 Bacteria; Cyanobacteriota
 Bacteria; Planctomycetota
 Bacteria; Pseudomonadota
 Archaea; Methanomicrobia
Fusion:
 Nter: <Unknown>
 Cter: None
Duplicate: in HAHCH, PSEA6, SHEAM, SHEB5, SHEB8, SHEB9, SHEDO, SHEFN, SHEON,
 SHEPC, SHESA, SHESM, SHESR, SHESW
Plasmid: None
Comments: The ribosomal protein bS6-modifying function does not apply to all
 RimK family members since some bacteria do not possess a rpsF gene in their
 genome, or the C-terminal sequence of RpsF is not conserved, or does not end
 with a glutamate residue.
XX
# Revision 1.25 2023/06/01
//