AC   MF_01617;
DC   Protein; auto
TR   HAMAP; MF_01617; -; 1; level=0
XX
Names: FadJ
XX
ID   FADJ
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadJ;
XX
CC   -!- FUNCTION: Catalyzes the formation of a hydroxyacyl-CoA by addition of
CC       water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-
CC       hydroxyacyl-CoA dehydrogenase activities.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC         NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA;
CC         Xref=Rhea:RHEA:21760, ChEBI:CHEBI:57315, ChEBI:CHEBI:57316;
CC         EC=5.1.2.3;
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (FadJ) and two beta chains
CC       (FadI).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC       dehydrogenase family.
XX
DR   PROSITE; PS00067; 3HCDH; 1; trigger=no
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1; trigger=no
DR   Pfam; PF00725; 3HCDH; 2; trigger=no
DR   Pfam; PF02737; 3HCDH_N; 1; trigger=no
DR   Pfam; PF00378; ECH; 1; trigger=no
DR   NCBIfam; TIGR02440; FadJ; 1; trigger=no
XX
KW   Cytoplasm
KW   Fatty acid metabolism
KW   Isomerase
KW   Lipid degradation
KW   Lipid metabolism
KW   Lyase
KW   Multifunctional enzyme
KW   NAD
KW   Oxidoreductase
XX
GO   GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity
GO   GO:0004300; F:enoyl-CoA hydratase activity
GO   GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity
GO   GO:0006631; P:fatty acid metabolic process
GO   GO:0016042; P:lipid catabolic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: FADJ_ECOLI (P77399)
FT   REGION          Nter..190
FT                   /note="Enoyl-CoA hydratase"
FT   REGION          306..Cter
FT                   /note="3-hydroxyacyl-CoA dehydrogenase"
FT   SITE            118
FT                   /note="Important for catalytic activity"
FT   Condition: E
FT   SITE            140
FT                   /note="Important for catalytic activity"
FT   Condition: E
XX
Size: 699-780;
Related: None;
Template: P77399;
Scope:
 Bacteria; Gammaproteobacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
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# Revision 1.29 2023/06/01
//