AC   MF_01619;
DC   Protein; auto
TR   HAMAP; MF_01619; -; 1; level=0
XX
Names: NAD_malic_enz
XX
ID   MAO1
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=maeA;
XX
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = CO2 + NADH + pyruvate;
CC         Xref=Rhea:RHEA:12653, ChEBI:CHEBI:15361, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.38;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + oxaloacetate = CO2 + pyruvate; Xref=Rhea:RHEA:15641,
CC         ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:16526; EC=1.1.1.38;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=Divalent metal cations. Prefers magnesium or manganese.;
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
XX
DR   PROSITE; PS00331; MALIC_ENZYMES; 1; trigger=no
DR   Pfam; PF00390; malic; 1; trigger=no
DR   Pfam; PF03949; Malic_M; 1; trigger=no
DR   PRINTS; PR00072; MALOXRDTASE; 1; trigger=no
XX
KW   Metal-binding
KW   NAD
KW   Oxidoreductase
XX
GO   GO:0004471; F:malate dehydrogenase (decarboxylating) (NAD+) activity
GO   GO:0008948; F:oxaloacetate decarboxylase activity
XX
FT   From: MAO1_ECOLI (P26616)
FT   ACT_SITE        104
FT                   /note="Proton donor"
FT   Condition: Y
FT   ACT_SITE        175
FT                   /note="Proton acceptor"
FT   Condition: K
FT   BINDING         246
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: E
FT   BINDING         247
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: D
FT   BINDING         270
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: D
FT   BINDING         157
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: R
FT   BINDING         270
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: D
FT   BINDING         418
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: N
FT   SITE            270
FT                   /note="Important for activity"
XX
Size: 528-591;
Related: None;
Template: P26616;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: in PHOPR, VIBVY
Plasmid: None
Comments: None
XX
# Revision 1.21 2023/01/26
//