AC   MF_01670;
DC   Protein; auto
TR   HAMAP; MF_01670; -; 1; level=0
XX
Names: IolA
XX
ID   IOLA
DE   RecName: Full=Malonate-semialdehyde dehydrogenase;
DE            Short=MSA dehydrogenase;
DE            EC=1.2.1.27;
DE   AltName: Full=Methylmalonate semialdehyde dehydrogenase;
DE            Short=MMSA dehydrogenase;
DE            Short=MSDH;
GN   Name=iolA;
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CC   -!- FUNCTION: Catalyzes the oxidation of malonate semialdehyde (MSA) and
CC       methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA,
CC       respectively. Is involved in a myo-inositol catabolic pathway.
CC       Bicarbonate, and not CO2, is the end-product of the enzymatic reaction.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxopropanoate + CoA + H2O + NAD(+) = acetyl-CoA + H(+) +
CC         hydrogencarbonate + NADH; Xref=Rhea:RHEA:76615, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:33190,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.27;
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:76616;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-methyl-3-oxopropanoate + CoA + H2O + NAD(+) = H(+) +
CC         hydrogencarbonate + NADH + propanoyl-CoA; Xref=Rhea:RHEA:20804,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57392, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57700, ChEBI:CHEBI:57945; EC=1.2.1.27;
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20805;
CC   -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC       acetyl-CoA from myo-inositol: step 7/7.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family. IolA
CC       subfamily.
XX
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1; trigger=no
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1; trigger=no
DR   Pfam; PF00171; Aldedh; 1; trigger=no
DR   NCBIfam; TIGR01722; MMSDH; 1; trigger=no
XX
KW   NAD
KW   Oxidoreductase
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GO   GO:0004491; F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity
GO   GO:0018478; F:malonate-semialdehyde dehydrogenase (acetylating) activity
GO   GO:0019310; P:inositol catabolic process
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FT   From: IOLA_BACSU (P42412)
FT   BINDING         150
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Optional; Condition: A
FT   BINDING         152
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: F
FT   BINDING         176
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: K
FT   BINDING         179
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: E
FT   BINDING         180
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: [KR]
FT   BINDING         229
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Optional; Condition: T
FT   BINDING         251
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: T
FT   BINDING         382
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: E
FT   ACT_SITE        284
FT                   /note="Nucleophile"
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Size: 470-520;
Related: None;
Template: P42412;
Scope:
 Bacteria; Bacillota
Fusion:
 Nter: None
 Cter: None
Duplicate: in BACAH, BACAN, BACCZ, BACHK, BACLD, SHOC1, BACMK, GEOKA, GEOTN,
 OCEIH
Plasmid: in BACCZ
Comments: None
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# Revision 1.17 2023/11/28
//