AC   MF_01675;
DC   Protein; auto
TR   HAMAP; MF_01675; -; 1; level=0
XX
Names: Sep_Cys_tRNA_synth
XX
ID   SPSS
DE   RecName: Full=O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
DE            EC=2.5.1.73;
DE   AltName: Full=Sep-tRNA:Cys-tRNA synthase;
DE            Short=SepCysS;
XX
CC   -!- FUNCTION: Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-
CC       cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-
CC         cysteinyl-tRNA(Cys) + phosphate; Xref=Rhea:RHEA:25686, Rhea:RHEA-
CC         COMP:9679, Rhea:RHEA-COMP:9719, ChEBI:CHEBI:15378, ChEBI:CHEBI:29919,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:78517, ChEBI:CHEBI:78551; EC=2.5.1.73;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC   -!- SUBUNIT: Homodimer. Interacts with SepRS.
CC   -!- SIMILARITY: Belongs to the SepCysS family.
XX
DR   Pfam; PF05889; SLA_LP_auto_ag; 1; trigger=no
DR   NCBIfam; TIGR02539; SepCysS; 1; trigger=no
XX
KW   Protein biosynthesis
KW   Pyridoxal phosphate
KW   Transferase
XX
GO   GO:0043766; F:Sep-tRNA:Cys-tRNA synthase activity
XX
FT   From: SPSS1_ARCFU (O30207)
FT   BINDING         78..79
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT   Condition: A-R
FT   BINDING         206..208
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT   Condition: S-G-H
FT   BINDING         183
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT   Condition: N
FT   MOD_RES         209
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT   Condition: K
XX
Size: 371-463;
Related: None;
Template: Q59072; O30207;
Scope:
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: in ARCFU, METB6, METBU, METHJ, METLZ, METAR
Plasmid: None
Comments: Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus and
 Methanopyrus kandleri use the SepRS?SepCysS pathway obligatorily for synthesis of
 Cys-tRNACys, whereas many others (for example, Methanococcus maripaludis and Archaeoglobus
 fulgidus) contain genes for both the CysRS and the SepRS?SepCysS pathways (PMID:16380427).
XX
# Revision 1.18 2023/06/01
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