AC MF_01679; DC Protein; auto TR HAMAP; MF_01679; -; 1; level=0 XX Names: Salvage_MtnW XX ID MTNW DE RecName: Full=2,3-diketo-5-methylthiopentyl-1-phosphate enolase; DE Short=DK-MTP-1-P enolase; DE EC=5.3.2.5; DE AltName: Full=RuBisCO-like protein; DE Short=RLP; GN Name=mtnW; XX CC -!- FUNCTION: Catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1- CC phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1- CC phosphate (HK-MTPenyl-1-P). CC -!- CATALYTIC ACTIVITY: CC Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate = 2-hydroxy-5- CC methylsulfanyl-3-oxopent-1-enyl phosphate; Xref=Rhea:RHEA:18769, CC ChEBI:CHEBI:58828, ChEBI:CHEBI:59505; EC=5.3.2.5; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Note=Binds 1 Mg(2+) ion per subunit.; CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage CC pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: CC step 3/6. CC -!- SUBUNIT: Homodimer. CC -!- MISCELLANEOUS: Has no RuBP-carboxylation activity. CC -!- SIMILARITY: Belongs to the RuBisCO large chain family. Type IV CC subfamily. XX DR PROSITE; PS00157; RUBISCO_LARGE; 1; trigger=no DR Pfam; PF00016; RuBisCO_large; 1; trigger=no DR NCBIfam; TIGR03332; Salvage_mtnW; 1; trigger=no XX KW Amino-acid biosynthesis KW Isomerase KW Magnesium KW Metal-binding KW Methionine biosynthesis XX GO GO:0000287; F:magnesium ion binding GO GO:0043715; F:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity GO GO:0019284; P:L-methionine salvage from S-adenosylmethionine XX FT From: MTNW_GEOKA (Q5L1E2) FT BINDING 173..176 FT /ligand="substrate" FT BINDING 359..360 FT /ligand="substrate" FT ACT_SITE 98 FT /note="Proton acceptor" FT Condition: K FT BINDING 173 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /note="via carbamate group" FT Condition: K FT BINDING 175 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT Condition: D FT BINDING 176 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT Condition: E FT BINDING 147 FT /ligand="substrate" FT Condition: K FT BINDING 264 FT /ligand="substrate" FT Condition: H FT BINDING 337 FT /ligand="substrate" FT Condition: G FT MOD_RES 173 FT /note="N6-carboxylysine" FT Condition: K XX Size: 374-414; Related: None; Template: O31666; Q5L1E2; A8YER2; Scope: Bacteria; Microcystis Bacteria; Bacillota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.20 2023/06/01 //