AC   MF_01690;
DC   Protein; auto
TR   HAMAP; MF_01690; -; 1; level=0
XX
Names: DapE
XX
ID   DAPE
DE   RecName: Full=Succinyl-diaminopimelate desuccinylase;
DE            Short=SDAP desuccinylase;
DE            EC=3.5.1.18;
DE   AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
GN   Name=dapE;
XX
CC   -!- FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic
CC       acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP),
CC       an intermediate involved in the bacterial biosynthesis of lysine and
CC       meso-diaminopimelic acid, an essential component of bacterial cell
CC       walls.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-
CC         2,6-diaminoheptanedioate + succinate; Xref=Rhea:RHEA:22608,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:30031, ChEBI:CHEBI:57609,
CC         ChEBI:CHEBI:58087; EC=3.5.1.18;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC       Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate
CC       (succinylase route): step 3/3.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. DapE subfamily.
XX
DR   PROSITE; PS00758; ARGE_DAPE_CPG2_1; 1; trigger=no
DR   PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1; trigger=no
DR   Pfam; PF07687; M20_dimer; 1; trigger=no
DR   Pfam; PF01546; Peptidase_M20; 1; trigger=no
DR   NCBIfam; TIGR01246; DapE_proteo; 1; trigger=no
DR   NCBIfam; TIGR01910; DapE-ArgE; 1; trigger=no
XX
KW   Amino-acid biosynthesis
KW   Cobalt
KW   Diaminopimelate biosynthesis
KW   Hydrolase
KW   Lysine biosynthesis
KW   Metal-binding
KW   Zinc
XX
GO   GO:0008270; F:zinc ion binding
GO   GO:0009014; F:succinyl-diaminopimelate desuccinylase activity
GO   GO:0050897; F:cobalt ion binding
GO   GO:0009089; P:lysine biosynthetic process via diaminopimelate
GO   GO:0019877; P:diaminopimelate biosynthetic process
XX
FT   From: DAPE_HAEIN (P44514)
FT   ACT_SITE        69
FT   Condition: D
FT   ACT_SITE        134
FT                   /note="Proton acceptor"
FT   Condition: E
FT   BINDING         67
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Condition: H
FT   BINDING         100
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Condition: D
FT   BINDING         100
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Condition: D
FT   BINDING         135
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Condition: E
FT   BINDING         163
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Condition: E
FT   BINDING         349
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Condition: H
XX
Size: 363-425;
Related: None;
Template: Q8ZN75; P44514; P0AED7; Q9JYL2; Q9KQ52;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: in ALTMD, SHELP, RUEST
Plasmid: in PSESY, RUEST
Comments: None
XX
# Revision 1.19 2023/06/01
//