AC   MF_01847;
DC   Protein; auto
TR   HAMAP; MF_01847; -; 1; level=0
XX
Names: HMP_PP_phosphat
XX
ID   COF
DE   RecName: Full=HMP-PP phosphatase;
DE            EC=3.6.1.-;
GN   Name=cof;
XX
CC   -!- FUNCTION: Catalyzes the hydrolysis of 4-amino-2-methyl-5-
CC       hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-methyl-5-
CC       hydroxymethylpyrimidine phosphate (HMP-P).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H2O = 4-
CC         amino-2-methyl-5-(phosphooxymethyl)pyrimidine + H(+) + phosphate;
CC         Xref=Rhea:RHEA:27914, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57841, ChEBI:CHEBI:58354;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof family.
XX
DR   PROSITE; PS01228; COF_1; 1; trigger=no
DR   PROSITE; PS01229; COF_2; 1; trigger=no
DR   Pfam; PF00702; Hydrolase; 1; trigger=no
DR   Pfam; PF08282; Hydrolase_3; 1; trigger=no
DR   NCBIfam; TIGR00099; Cof-subfamily; 1; trigger=no
DR   NCBIfam; TIGR01484; HAD-SF-IIB; 1; trigger=no
XX
KW   Hydrolase
KW   Magnesium
KW   Metal-binding
XX
GO   GO:0000287; F:magnesium ion binding
GO   GO:0016791; F:phosphatase activity
XX
FT   From: COF_ECOLI (P46891)
FT   ACT_SITE        8
FT                   /note="Nucleophile"
FT   Condition: D
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         10
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         212
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
XX
Size: 260-274;
Related: None;
Template: P46891;
Scope:
 Bacteria; Enterobacterales
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: The N-terminus of ECOLC is shorter. SHIDS is truncated and probably not functional.
 These sequences have not been included in the alignment.
XX
# Revision 1.18 2023/06/01
//