AC   MF_01864;
DC   Protein; auto
TR   HAMAP; MF_01864; -; 1; level=0
XX
Names: tRNA_metthiotr_MiaB
XX
ID   MIAB
DE   RecName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase;
DE            EC=2.8.4.3;
DE   AltName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
DE   AltName: Full=tRNA-i(6)A37 methylthiotransferase;
GN   Name=miaB;
XX
CC   -!- FUNCTION: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine
CC       (i(6)A), leading to the formation of 2-methylthio-N6-
CC       (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read
CC       codons beginning with uridine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in
CC         tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-
CC         dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur
CC         carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:37067, Rhea:RHEA-COMP:10375, Rhea:RHEA-COMP:10376,
CC         Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:29917, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:74415,
CC         ChEBI:CHEBI:74417; EC=2.8.4.3;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC       subfamily.
XX
DR   PROSITE; PS50926; TRAM; 0-1; trigger=yes
DR   PROSITE; PS01278; MTTASE_RADICAL; 1; trigger=no
DR   PROSITE; PS51449; MTTASE_N; 1; trigger=yes
DR   PROSITE; PS51918; RADICAL_SAM; 1; trigger=yes
DR   Pfam; PF04055; Radical_SAM; 1; trigger=no
DR   Pfam; PF01938; TRAM; 0-1; trigger=no
DR   Pfam; PF00919; UPF0004; 1; trigger=no
DR   NCBIfam; TIGR01574; MiaB-methiolase; 1; trigger=no
DR   NCBIfam; TIGR00089; TIGR00089; 1; trigger=no
XX
KW   4Fe-4S
KW   Cytoplasm
KW   Iron
KW   Iron-sulfur
KW   Metal-binding
KW   S-adenosyl-L-methionine
KW   Transferase
KW   tRNA processing
XX
GO   GO:0016740; F:transferase activity
GO   GO:0051539; F:4 iron, 4 sulfur cluster binding
GO   GO:0006400; P:tRNA modification
GO   GO:0005737; C:cytoplasm
XX
FT   From: MIAB_ECOLI (P0AEI1)
FT   BINDING         12
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Group: 1; Condition: C
FT   BINDING         49
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Group: 1; Condition: C
FT   BINDING         83
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Group: 1; Condition: C
FT   BINDING         157
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 2; Condition: C
FT   BINDING         161
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 2; Condition: C
FT   BINDING         164
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 2; Condition: C
XX
Size: 418-540;
Related: None;
Template: P0AEI1; Q9RCI2; Q9WZC1; O31778;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: <Unknown>
Duplicate: in MYCMM
Plasmid: in CERS5, RUEPO
Comments: None
XX
# Revision 1.20 2023/06/01
//