AC   MF_01873;
DC   Protein; auto
TR   HAMAP; MF_01873; -; 1; level=0
XX
Names: 23SrRNA_methyltr_Cfr
XX
ID   CFR
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase Cfr;
DE            EC=2.1.1.224;
DE   AltName: Full=23S rRNA (adenine(2503)-C(8))-methyltransferase;
DE   AltName: Full=23S rRNA m8A2503 methyltransferase;
GN   Name=cfr;
XX
CC   -!- FUNCTION: Specifically methylates position 8 of adenine 2503 in 23S
CC       rRNA. Confers resistance to some classes of antibiotics.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin]
CC         + 2 S-adenosyl-L-methionine = 5'-deoxyadenosine + 8-
CC         methyladenosine(2503) in 23S rRNA + L-methionine + 2 oxidized [2Fe-
CC         2S]-[ferredoxin] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42632,
CC         Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10152,
CC         Rhea:RHEA-COMP:10153, ChEBI:CHEBI:17319, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74543;
CC         EC=2.1.1.224;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Reaction proceeds by a ping-pong mechanism involving
CC       intermediate methylation of a conserved cysteine residue.
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. RlmN family. Cfr
CC       subfamily.
XX
DR   Pfam; PF04055; Radical_SAM; 1; trigger=no
DR   PIRSF; PIRSF006004; CHP00048; 1; trigger=no
DR   NCBIfam; TIGR04432; rSAM_Cfr; 1; trigger=no
XX
KW   Antibiotic resistance
KW   Cytoplasm
KW   Disulfide bond
KW   Methyltransferase
KW   rRNA processing
KW   S-adenosyl-L-methionine
KW   Transferase
case <FTGroup:1>
KW   4Fe-4S
KW   Iron
KW   Iron-sulfur
KW   Metal-binding
end case
XX
GO   GO:0019843; F:rRNA binding
GO   GO:0016433; F:rRNA (adenine) methyltransferase activity
case <FTGroup:1>
GO   GO:0051539; F:4 iron, 4 sulfur cluster binding
end case
GO   GO:0070475; P:rRNA base methylation
GO   GO:0005737; C:cytoplasm
XX
FT   From: CFR_MAMSC (Q9FBG4)
FT   BINDING         158..159
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G-E
FT   BINDING         212..214
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: S-x-H
FT   ACT_SITE        91
FT                   /note="Proton acceptor"
FT   Condition: E
FT   ACT_SITE        338
FT                   /note="S-methylcysteine intermediate"
FT   Condition: C
FT   BINDING         112
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         116
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         119
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         189
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: S
FT   BINDING         293
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: N
FT   DISULFID        105..338
FT                   /note="(transient)"
FT   Condition: C-x*-C
XX
Size: 343-350;
Related: MF_01849;
Template: Q9FBG4; A5HBL2;
Scope:
 Bacteria; Bacillota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: in STAAU, STASC, STAWA
Comments: None
XX
# Revision 1.20 2023/06/01
//