AC MF_01892; DC Protein; auto TR HAMAP; MF_01892; -; 1; level=0 XX Names: tRNA_Ile2_agm2C_synt XX ID TIAS DE RecName: Full=tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS; DE Short=tRNA(Ile2)-agm2C synthetase; DE EC=6.3.4.22; DE AltName: Full=tRNA(Ile2) agmatidine synthetase; GN Name=tiaS; XX CC -!- FUNCTION: ATP-dependent agmatine transferase that catalyzes the CC formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) CC of tRNA(Ile2), converting the codon specificity from AUG to AUA. CC -!- CATALYTIC ACTIVITY: CC Reaction=agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2- CC agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate; CC Xref=Rhea:RHEA:43608, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10626, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58145, ChEBI:CHEBI:82748, CC ChEBI:CHEBI:83545, ChEBI:CHEBI:456215; EC=6.3.4.22; CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the TiaS family. XX DR Pfam; PF08489; DUF1743; 1; trigger=no DR Pfam; PF01336; tRNA_anti; 0-1; trigger=yes XX KW ATP-binding KW Nucleotide-binding KW Cytoplasm KW Ligase KW tRNA processing XX GO GO:0016879; F:ligase activity, forming carbon-nitrogen bonds GO GO:0002101; P:tRNA wobble cytosine modification XX FT None XX Size: 397-508; Related: None; Template: O28025; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: in ACIB4 Plasmid: None Comments: None XX # Revision 1.6 2019/11/19 //