AC MF_01921; DC Protein; auto TR HAMAP; MF_01921; -; 1; level=0 XX Names: TYW1_archaea XX ID TYW1 DE RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; DE EC=4.1.3.44; DE AltName: Full=tRNA wyosine derivatives biosynthesis protein Taw1; GN Name=taw1; XX CC -!- FUNCTION: Component of the wyosine derivatives biosynthesis pathway CC that catalyzes the condensation of N-methylguanine with 2 carbon atoms CC from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on CC guanosine-37 of tRNA(Phe). CC -!- CATALYTIC ACTIVITY: CC Reaction=N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl- CC L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine CC + CO2 + H2O + L-methionine; Xref=Rhea:RHEA:36347, Rhea:RHEA- CC COMP:10164, Rhea:RHEA-COMP:10165, ChEBI:CHEBI:15361, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16526, ChEBI:CHEBI:17319, CC ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315, CC ChEBI:CHEBI:73542; EC=4.1.3.44; case CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated CC with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; end case CC -!- SUBUNIT: Monomer. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the TYW1 family. XX DR Pfam; PF04055; Radical_SAM; 1; trigger=no DR Pfam; PF08608; Wyosine_form; 1; trigger=no DR NCBIfam; TIGR03972; rSAM_TYW1; 1; trigger=no XX case KW 4Fe-4S KW Iron KW Iron-sulfur KW Metal-binding end case KW Cytoplasm KW Lyase KW S-adenosyl-L-methionine KW tRNA processing XX case GO GO:0051539; F:4 iron, 4 sulfur cluster binding end case GO GO:0016833; F:oxo-acid-lyase activity GO GO:0008033; P:tRNA processing GO GO:0005737; C:cytoplasm XX FT From: TYW1_METJA (Q57705) FT BINDING 26 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 39 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 52 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 62 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT /ligand_note="4Fe-4S-S-AdoMet" FT Group: 1; Condition: C FT BINDING 66 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT /ligand_note="4Fe-4S-S-AdoMet" FT Group: 1; Condition: C FT BINDING 69 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT /ligand_note="4Fe-4S-S-AdoMet" FT Group: 1; Condition: C XX Size: 288-386; Related: None; Template: Q57705; Q9V1F9; O59412; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.9 2023/06/01 //