AC   MF_01953;
DC   Protein; auto
TR   HAMAP; MF_01953; -; 1; level=0
XX
Names: Urease_alpha
XX
ID   URE1
case not <OC:Campylobacterales>
DE   RecName: Full=Urease subunit alpha;
DE            EC=3.5.1.5;
DE   AltName: Full=Urea amidohydrolase subunit alpha;
GN   Name=ureC;
else case <OC:Campylobacterales>
DE   RecName: Full=Urease subunit beta;
DE            EC=3.5.1.5;
DE   AltName: Full=Urea amidohydrolase subunit beta;
GN   Name=ureB;
end case
XX
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
case <FTGroup:1> and <FTGroup:2>
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC       Note=Binds 2 nickel ions per subunit.;
end case
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC       urea (urease route): step 1/1.
case <OC:Campylobacterales>
CC   -!- SUBUNIT: Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits.
else case <OC:Deinococcus> or <OC:Sulfolobus>
CC   -!- SUBUNIT: Heterohexamer of 3 UreC (alpha) and 3 UreAB (gamma/beta)
CC       subunits.
else case <OS:Pseudomonas syringae> or <OC:Streptomyces>
CC   -!- SUBUNIT: May form a heterohexamer of 3 UreC (alpha) and 3 UreAB
CC       (gamma/beta) subunits. May also form a heterotrimer of UreA (gamma),
CC       UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to
CC       form the active enzyme.
else
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC       subunits. Three heterotrimers associate to form the active enzyme.
end case
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
case <FT:4>
CC   -!- PTM: Carboxylation allows a single lysine to coordinate two nickel
CC       ions.
end case
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Urease alpha subunit family.
case <OC:Campylobacterales>
CC   -!- CAUTION: The orthologous protein is known as the alpha subunit (UreC)
CC       in most other bacteria.
end case
XX
DR   PROSITE; PS01120; UREASE_1; 1; trigger=no
DR   PROSITE; PS00145; UREASE_2; 1; trigger=no
DR   PROSITE; PS51368; UREASE_3; 1; trigger=yes
DR   Pfam; PF01979; Amidohydro_1; 1; trigger=no
DR   Pfam; PF00449; Urease_alpha; 1; trigger=no
DR   PRINTS; PR01752; UREASE; 1; trigger=no
DR   NCBIfam; TIGR01792; Urease_alph; 1; trigger=no
XX
KW   Cytoplasm
KW   Hydrolase
case <FTGroup:1> or <FTGroup:2>
KW   Metal-binding
KW   Nickel
end case
XX
GO   GO:0009039; F:urease activity
case <FTGroup:1> or <FTGroup:2>
GO   GO:0016151; F:nickel cation binding
end case
GO   GO:0019627; P:urea metabolic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: URE1_KLEAE (P18314)
FT   ACT_SITE        320
FT                   /note="Proton donor"
FT   Condition: H
FT   BINDING         134
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT   Group: 2; Condition: H
FT   BINDING         136
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT   Group: 2; Condition: H
FT   BINDING         217
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /note="via carbamate group"
FT   Group: 1; Condition: K
FT   BINDING         217
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /note="via carbamate group"
FT   Group: 2; Condition: K
FT   BINDING         246
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT   Group: 1; Condition: H
FT   BINDING         272
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT   Group: 1; Condition: H
FT   BINDING         360
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT   Group: 2; Condition: D
FT   BINDING         219
FT                   /ligand="substrate"
FT   Condition: H
FT   MOD_RES         217
FT                   /note="N6-carboxylysine"
FT   Condition: K
XX
Size: 556-598;
Related: None;
Template: P18314; P77837; P69996;
Scope:
 Bacteria
 Archaea; Sulfolobales
 Archaea; Halobacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in BRUA2, BRUAB, BRUME, BRUSU, ECO57, PSEU2, STRAW, STRCO
Plasmid: in CLOPE, ECOLX, HALMA
Comments: None
XX
# Revision 1.36 2023/06/01
//