AC   MF_02019;
DC   Protein; auto
TR   HAMAP; MF_02019; -; 1; level=0
XX
Names: MurF
XX
ID   MURF
DE   RecName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase;
DE            EC=6.3.2.10;
DE   AltName: Full=D-alanyl-D-alanine-adding enzyme;
GN   Name=murF;
XX
CC   -!- FUNCTION: Involved in cell wall formation. Catalyzes the final step in
CC       the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of
CC       murein.
case <OC:Lactobacillales> or <OC:Staphylococcus>
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-
CC         alanyl-gamma-D-glutamyl-L-lysine = ADP + H(+) + phosphate + UDP-N-
CC         acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-
CC         alanine; Xref=Rhea:RHEA:16085, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57822, ChEBI:CHEBI:70758,
CC         ChEBI:CHEBI:83903, ChEBI:CHEBI:456216; EC=6.3.2.10;
else
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-
CC         alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) +
CC         phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-
CC         meso-2,6-diaminopimeloyl-D-alanyl-D-alanine; Xref=Rhea:RHEA:28374,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57822, ChEBI:CHEBI:61386, ChEBI:CHEBI:83905,
CC         ChEBI:CHEBI:456216; EC=6.3.2.10;
end case
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the MurCDEF family. MurF subfamily.
XX
DR   Pfam; PF01225; Mur_ligase; 1; trigger=no
DR   Pfam; PF02875; Mur_ligase_C; 1; trigger=no
DR   Pfam; PF08245; Mur_ligase_M; 1; trigger=no
DR   NCBIfam; TIGR01143; murF; 1; trigger=no
XX
KW   ATP-binding
KW   Cell cycle
KW   Cell division
KW   Cell shape
KW   Cell wall biogenesis/degradation
KW   Cytoplasm
KW   Ligase
KW   Nucleotide-binding
KW   Peptidoglycan synthesis
XX
GO   GO:0005524; F:ATP binding
GO   GO:0047480; F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity
GO   GO:0009252; P:peptidoglycan biosynthetic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: MURF_ECOLI (P11880)
FT   BINDING         107..113
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: G-[ST]-x-G-K-T-[ST]
XX
Size: 420-535;
Related: None;
Template: P11880; Q2FWH4;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.8 2023/06/01
//