AC   MF_02029;
DC   Protein; auto
TR   HAMAP; MF_02029; -; 1; level=0
XX
Names: WecC_RffD
XX
ID   WECC
DE   RecName: Full=UDP-N-acetyl-D-mannosamine dehydrogenase;
DE            EC=1.1.1.336;
DE   AltName: Full=UDP-ManNAc 6-dehydrogenase;
GN   Name=wecC;
XX
CC   -!- FUNCTION: Catalyzes the four-electron oxidation of UDP-N-acetyl-D-
CC       mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N-
CC       acetylmannosaminuronic acid (UDP-ManNAcA).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2
CC         NADH + UDP-N-acetyl-alpha-D-mannosaminouronate; Xref=Rhea:RHEA:25780,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:68623, ChEBI:CHEBI:70731;
CC         EC=1.1.1.336;
CC   -!- SUBUNIT: Homodimer.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis.
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family. WecC subfamily.
XX
DR   Pfam; PF00984; UDPG_MGDP_dh; 1; trigger=no
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1; trigger=no
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1; trigger=no
DR   NCBIfam; TIGR03026; NDP-sugDHase; 1; trigger=no
DR   PIRSF; PIRSF500136; UDP_ManNAc_DH; 1; trigger=no
DR   PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1; trigger=no
XX
KW   NAD
KW   Oxidoreductase
XX
GO   GO:0089714; F:UDP-N-acetyl-D-mannosamine dehydrogenase activity
GO   GO:0009246; P:enterobacterial common antigen biosynthetic process
XX
FT   From: WECC_ECOLI (P27829)
FT   ACT_SITE        212
FT                   /note="Proton donor/acceptor"
FT   Condition: K
FT   ACT_SITE        266
FT                   /note="Nucleophile"
FT   Condition: C
FT   BINDING         13
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: Y
FT   BINDING         14
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: I
FT   BINDING         33
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: D
FT   BINDING         85
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: T
FT   BINDING         126
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: T
FT   BINDING         160
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: R
FT   BINDING         161
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: V
FT   BINDING         212
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: K
FT   BINDING         216
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: N
FT   BINDING         219
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: R
FT   BINDING         250
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain B"
FT   Condition: H
FT   BINDING         252
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain B"
FT   Condition: R
FT   BINDING         263
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: G
FT   BINDING         330
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: [FY]
FT   BINDING         331
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: K
FT   BINDING         338
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain B"
FT   Condition: R
FT   BINDING         416
FT                   /ligand="UDP-N-acetyl-alpha-D-mannosaminouronate"
FT                   /ligand_id="ChEBI:CHEBI:70731"
FT                   /ligand_note="ligand shared between homodimeric partners"
FT                   /note="in chain A"
FT   Condition: [RK]
XX
Size: 418-445;
Related: None;
Template: P27829;
Scope:
 Bacteria; Enterobacterales
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.11 2023/06/01
//