AC   MF_02041;
DC   Protein; auto
TR   HAMAP; MF_02041; -; 1; level=0
XX
Names: DusA_subfam
XX
case <OC:Pseudomonadota>
ID   DUSA
DE   RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
DE            EC=1.3.1.-;
DE            EC=1.3.1.91;
DE   AltName: Full=U20-specific dihydrouridine synthase;
DE            Short=U20-specific Dus;
DE   AltName: Full=tRNA-dihydrouridine synthase A;
GN   Name=dusA;
else
ID   DUSAL
DE   RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
DE            EC=1.3.1.-;
DE            EC=1.3.1.91;
DE   AltName: Full=DusA-like U20-specific dihydrouridine synthase;
DE            Short=U20-specific Dus;
end case
XX
CC   -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified
CC       base found in the D-loop of most tRNAs, via the reduction of the C5-C6
CC       double bond in target uridines. Specifically modifies U20 and U20a in
CC       tRNAs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH +
CC         uridine(20) in tRNA; Xref=Rhea:RHEA:53336, Rhea:RHEA-COMP:13533,
CC         Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH +
CC         uridine(20) in tRNA; Xref=Rhea:RHEA:53340, Rhea:RHEA-COMP:13533,
CC         Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH +
CC         uridine(20a) in tRNA; Xref=Rhea:RHEA:53344, Rhea:RHEA-COMP:13535,
CC         Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH +
CC         uridine(20a) in tRNA; Xref=Rhea:RHEA:53348, Rhea:RHEA-COMP:13535,
CC         Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the Dus family. DusA subfamily.
XX
DR   PROSITE; PS01136; UPF0034; 1; trigger=no
DR   Pfam; PF01207; Dus; 1; trigger=no
DR   NCBIfam; TIGR00742; yjbN; 1; trigger=no
DR   PIRSF; PIRSF006621; Dus; 1; trigger=no
XX
KW   Flavoprotein
KW   FMN
KW   NADP
KW   Oxidoreductase
KW   RNA-binding
KW   tRNA processing
KW   tRNA-binding
XX
GO   GO:0010181; F:FMN binding
GO   GO:0000049; F:tRNA binding
GO   GO:0017150; F:tRNA dihydrouridine synthase activity
GO   GO:0002943; P:tRNA dihydrouridine synthesis
XX
FT   From: DUSAL_THET8 (Q5SMC7)
FT   BINDING         10..12
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: P-M-[VML]
FT   BINDING         203..205
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: N-G-G
FT   BINDING         225..226
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: G-R
FT   ACT_SITE        93
FT                   /note="Proton donor"
FT   Condition: C
FT   BINDING         63
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: Q
FT   BINDING         132
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: K
FT   BINDING         164
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: H
FT   SITE            90
FT                   /note="Interacts with tRNA"
FT   Optional; Condition: N
FT   SITE            175
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: K
FT   SITE            178
FT                   /note="Interacts with tRNA"
FT   Optional; Condition: R
FT   SITE            290
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: R
FT   SITE            293
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: R
XX
Size: 288-400;
Related: None;
Template: Q5SMC7; P32695;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.7 2023/06/01
//