AC   MF_02042;
DC   Protein; auto
TR   HAMAP; MF_02042; -; 1; level=0
XX
Names: DusB_subfam
XX
ID   DUSB
DE   RecName: Full=tRNA-dihydrouridine synthase B;
DE            EC=1.3.1.-;
GN   Name=dusB;
XX
CC   -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified
CC       base found in the D-loop of most tRNAs, via the reduction of the C5-C6
CC       double bond in target uridines.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA +
CC         H(+) + NADH; Xref=Rhea:RHEA:54452, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA +
CC         H(+) + NADPH; Xref=Rhea:RHEA:23624, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:13887, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the Dus family. DusB subfamily.
XX
DR   PROSITE; PS01136; UPF0034; 1; trigger=no
DR   Pfam; PF01207; Dus; 1; trigger=no
DR   NCBIfam; TIGR00737; nifR3_yhdG; 1; trigger=no
DR   PIRSF; PIRSF006621; Dus; 1; trigger=no
XX
KW   Flavoprotein
KW   FMN
KW   NADP
KW   Oxidoreductase
KW   RNA-binding
KW   tRNA processing
KW   tRNA-binding
XX
GO   GO:0010181; F:FMN binding
GO   GO:0000049; F:tRNA binding
GO   GO:0017150; F:tRNA dihydrouridine synthase activity
GO   GO:0002943; P:tRNA dihydrouridine synthesis
XX
FT   From: DUSB_ECOLI (P0ABT5)
FT   BINDING         16..18
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: P-M-A
FT   BINDING         200..202
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: N-G-D
FT   BINDING         224..225
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: G-R
FT   ACT_SITE        100
FT                   /note="Proton donor"
FT   Condition: C
FT   BINDING         70
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: Q
FT   BINDING         139
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: K
XX
Size: 305-400;
Related: None;
Template: P0ABT5;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.7 2023/06/01
//