AC   MF_02043;
DC   Protein; auto
TR   HAMAP; MF_02043; -; 1; level=0
XX
Names: DusC_subfam
XX
ID   DUSC
DE   RecName: Full=tRNA-dihydrouridine(16) synthase;
DE            EC=1.3.1.-;
DE   AltName: Full=U16-specific dihydrouridine synthase;
DE            Short=U16-specific Dus;
DE   AltName: Full=tRNA-dihydrouridine synthase C;
GN   Name=dusC;
XX
CC   -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified
CC       base found in the D-loop of most tRNAs, via the reduction of the C5-C6
CC       double bond in target uridines. Specifically modifies U16 in tRNAs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH +
CC         uridine(16) in tRNA; Xref=Rhea:RHEA:53376, Rhea:RHEA-COMP:13543,
CC         Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH +
CC         uridine(16) in tRNA; Xref=Rhea:RHEA:53380, Rhea:RHEA-COMP:13543,
CC         Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- SIMILARITY: Belongs to the Dus family. DusC subfamily.
XX
DR   PROSITE; PS01136; UPF0034; 1; trigger=no
DR   Pfam; PF01207; Dus; 1; trigger=no
DR   PIRSF; PIRSF006621; Dus; 1; trigger=no
XX
KW   Flavoprotein
KW   FMN
KW   NADP
KW   Oxidoreductase
KW   RNA-binding
KW   tRNA processing
KW   tRNA-binding
XX
GO   GO:0010181; F:FMN binding
GO   GO:0000049; F:tRNA binding
GO   GO:0017150; F:tRNA dihydrouridine synthase activity
GO   GO:0002943; P:tRNA dihydrouridine synthesis
XX
FT   From: DUSC_ECOLI (P33371)
FT   BINDING         7..9
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: P-M-[EQ]
FT   BINDING         200..202
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: N-G-[ED]
FT   BINDING         224..225
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: [GC]-[RC]
FT   ACT_SITE        98
FT                   /note="Proton donor"
FT   Condition: C
FT   BINDING         68
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: Q
FT   BINDING         139
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT   Optional; Condition: [KR]
FT   SITE            35
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: R
FT   SITE            95
FT                   /note="Interacts with tRNA"
FT   Optional; Condition: N
FT   SITE            176
FT                   /note="Interacts with tRNA"
FT   Optional; Condition: Y
FT   SITE            272
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: R
FT   SITE            274
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: K
FT   SITE            279
FT                   /note="Interacts with tRNA"
FT   Optional; Condition: Y
FT   SITE            295
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT   Optional; Condition: [RK]
XX
Size: 290-360;
Related: None;
Template: P33371;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.6 2023/01/26
//