AC   MF_02059;
DC   Protein; auto
TR   HAMAP; MF_02059; -; 1; level=0
XX
Names: Activ_enz_CutD
XX
ID   CUTD
DE   RecName: Full=Choline trimethylamine-lyase activating enzyme;
DE            EC=1.97.1.-;
DE   AltName: Full=Choline utilization protein D;
DE   AltName: Full=GRE activase CutD;
DE   AltName: Full=Glycyl-radical enzyme activating enzyme CutD;
DE            Short=GRE activating enzyme CutD;
GN   Name=cutD;
XX
CC   -!- FUNCTION: Catalyzes activation of the choline trimethylamine-lyase CutC
CC       under anaerobic conditions by generation of an organic free radical on
CC       a glycine residue, via an homolytic cleavage of S-adenosyl-L-methionine
CC       (SAM).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-
CC         methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+)
CC         + L-methionine + semiquinone [flavodoxin]; Xref=Rhea:RHEA:61976,
CC         Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:14480, Rhea:RHEA-COMP:15993,
CC         Rhea:RHEA-COMP:15994, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319,
CC         ChEBI:CHEBI:29947, ChEBI:CHEBI:32722, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:140311;
case <FTGroup:1> and <FTGroup:2>
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine;
else
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
CC   -!- PATHWAY: Amine and polyamine metabolism; choline degradation.
CC   -!- SIMILARITY: Belongs to the organic radical-activating enzymes family.
XX
DR   PROSITE; PS00198; 4FE4S_FER_1; 2; trigger=no
DR   PROSITE; PS51379; 4FE4S_FER_2; 2; trigger=yes
DR   PROSITE; PS01087; RADICAL_ACTIVATING; 1; trigger=no
DR   Pfam; PF04055; Radical_SAM; 1; trigger=no
DR   NCBIfam; TIGR04395; cutC_activ_rSAM; 1; trigger=no
DR   NCBIfam; TIGR02494; PFLE_PFLC; 1; trigger=no
DR   PIRSF; PIRSF000371; PFL_act_enz; 1; trigger=no
XX
KW   4Fe-4S
KW   Iron
KW   Iron-sulfur
KW   Metal-binding
KW   Oxidoreductase
KW   Repeat
KW   S-adenosyl-L-methionine
XX
GO   GO:0016491; F:oxidoreductase activity
GO   GO:0051539; F:4 iron, 4 sulfur cluster binding
GO   GO:0042426; P:choline catabolic process
XX
FT   From: CUTD_OLEA2 (Q30W71)
FT   BINDING         37..39
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: W-C-[SA]
FT   BINDING         188..190
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: D-[VLIM]-K
FT   BINDING         31
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         35
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         38
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         57
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Group: 2; Condition: C
FT   BINDING         60
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Group: 2; Condition: C
FT   BINDING         63
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Group: 2; Condition: C
FT   BINDING         99
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Group: 2; Condition: C
FT   BINDING         139
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G
FT   BINDING         264
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: H
XX
Size: 300-360;
Related: None;
Template: Q30W71;
Scope:
 Bacteria; Coriobacteriales
 Bacteria; Bacillota
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.11 2023/06/01
//