AC   MF_02090;
DC   Protein; auto
TR   HAMAP; MF_02090; -; 1; level=0
XX
Names: NadE_glutamine_dep
XX
ID   NADE
DE   RecName: Full=Glutamine-dependent NAD(+) synthetase;
DE            EC=6.3.5.1;
DE   AltName: Full=NAD(+) synthase [glutamine-hydrolyzing];
GN   Name=nadE;
XX
CC   -!- FUNCTION: Catalyzes the ATP-dependent amidation of deamido-NAD to form
CC       NAD. Uses L-glutamine as a nitrogen source.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate +
CC         H(+) + L-glutamate + NAD(+); Xref=Rhea:RHEA:24384, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58359,
CC         ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.5.1;
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (L-Gln route): step 1/1.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the NAD synthetase
CC       family.
XX
DR   PROSITE; PS50263; CN_HYDROLASE; 1; trigger=yes
DR   Pfam; PF00795; CN_hydrolase; 1; trigger=no
DR   Pfam; PF02540; NAD_synthase; 1; trigger=no
DR   NCBIfam; TIGR00552; nadE; 1; trigger=no
DR   PIRSF; PIRSF006630; NADS_GAT; 1; trigger=no
XX
KW   ATP-binding
KW   Ligase
KW   NAD
KW   Nucleotide-binding
XX
GO   GO:0005524; F:ATP binding
GO   GO:0008795; F:NAD+ synthase activity
GO   GO:0009435; P:NAD biosynthetic process
XX
FT   From: NADE_MYCTU (P9WJJ3)
FT   BINDING         366..373
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: [GAP]-x-S-G-G-x-D-S
FT   BINDING         490..493
FT                   /ligand="deamido-NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:58437"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: W-x-T-[YF]
FT   ACT_SITE        52
FT                   /note="Proton acceptor; for glutaminase activity"
FT   Condition: E
FT   ACT_SITE        121
FT                   /note="For glutaminase activity"
FT   Condition: K
FT   ACT_SITE        176
FT                   /note="Nucleophile; for glutaminase activity"
FT   Condition: C
FT   BINDING         127
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Optional; Condition: Y
FT   BINDING         203
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Optional; Condition: S
FT   BINDING         209
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT   Optional; Condition: [RK]
FT   BINDING         456
FT                   /ligand="deamido-NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:58437"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: N
FT   BINDING         480
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: T
FT   BINDING         485
FT                   /ligand="deamido-NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:58437"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: E
FT   BINDING         635
FT                   /ligand="deamido-NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:58437"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: K
XX
Size: 510-735;
Related: None;
Template: P9WJJ3;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.8 2023/06/01
//