AC MF_02106; DC Protein; auto TR HAMAP; MF_02106; -; 1; level=0 XX Names: Pup XX ID PUP DE RecName: Full=Prokaryotic ubiquitin-like protein Pup; DE AltName: Full=Bacterial ubiquitin-like modifier; GN Name=pup; XX CC -!- FUNCTION: Protein modifier that is covalently attached to lysine CC residues of substrate proteins, thereby targeting them for proteasomal CC degradation. The tagging system is termed pupylation. CC -!- PATHWAY: Protein degradation; proteasomal Pup-dependent pathway. CC -!- SUBUNIT: Strongly interacts with the proteasome-associated ATPase ARC CC through a hydrophobic interface; the interacting region of Pup lies in CC its C-terminal half. There is one Pup binding site per ARC hexamer CC ring. CC -!- DOMAIN: The N-terminal unstructured half of Pup provides a signal CC required to initiate unfolding and degradation by the proteasome but is CC not needed for pupylation, while the C-terminal helical half of Pup CC interacts with ARC to target proteins to the proteasome. case CC -!- PTM: Is modified by deamidation of its C-terminal glutamine to CC glutamate by the deamidase Dop, a prerequisite to the subsequent CC pupylation process. end case CC -!- SIMILARITY: Belongs to the prokaryotic ubiquitin-like protein family. XX DR Pfam; PF05639; Pup; 1; trigger=no DR General; Coiled_coil; -; 0-unlimited; trigger=yes XX KW Isopeptide bond KW Ubl conjugation pathway XX GO GO:0031386; F:protein tag activity GO GO:0070628; F:proteasome binding GO GO:0010498; P:proteasomal protein catabolic process GO GO:0019941; P:modification-dependent protein catabolic process GO GO:0070490; P:protein pupylation XX FT From: PUP_MYCTU (P9WHN5) FT REGION 21..58 FT /note="ARC ATPase binding" FT MOD_RES 64 FT /note="Deamidated glutamine" FT Tag: deamidation; Optional; Condition: Q case FT CROSSLNK 64 FT /note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain FT with K-? in acceptor proteins)" FT Condition: [Q] else FT CROSSLNK 64 FT /note="Isoglutamyl lysine isopeptide (Glu-Lys) (interchain FT with K-? in acceptor proteins)" FT Condition: [E] end case XX Size: 62-72; Related: None; Template: P9WHN5; A0QZ48; Q8NQE0; Scope: Bacteria; Actinomycetota Fusion: Nter: None Cter: None Duplicate: in RHOER, SACEN Plasmid: None Comments: Pup protein sequences end with either a GGQ motif or a GGE motif. Those ending by GGQ must be deaminated into GGE by the deamidase Dop to be able to link the protein target during the pupylation process. XX # Revision 1.12 2023/07/27 //