AC   MF_02111;
DC   Protein; auto
TR   HAMAP; MF_02111; -; 1; level=0
XX
Names: Pup_ligase
XX
ID   PAFA
DE   RecName: Full=Pup--protein ligase;
DE            EC=6.3.1.19;
DE   AltName: Full=Proteasome accessory factor A;
DE   AltName: Full=Pup-conjugating enzyme;
GN   Name=pafA;
XX
CC   -!- FUNCTION: Catalyzes the covalent attachment of the prokaryotic
CC       ubiquitin-like protein modifier Pup to the proteasomal substrate
CC       proteins, thereby targeting them for proteasomal degradation. This
CC       tagging system is termed pupylation. The ligation reaction involves the
CC       side-chain carboxylate of the C-terminal glutamate of Pup and the side-
CC       chain amino group of a substrate lysine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + [prokaryotic ubiquitin-like protein]-L-glutamate +
CC         [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin-
CC         like protein]-gamma-L-glutamyl)-[protein]-L-lysine.; EC=6.3.1.19;
CC   -!- PATHWAY: Protein degradation; proteasomal Pup-dependent pathway.
CC   -!- PATHWAY: Protein modification; protein pupylation.
CC   -!- MISCELLANEOUS: The reaction mechanism probably proceeds via the
CC       activation of Pup by phosphorylation of its C-terminal glutamate, which
CC       is then subject to nucleophilic attack by the substrate lysine,
CC       resulting in an isopeptide bond and the release of phosphate as a good
CC       leaving group.
CC   -!- SIMILARITY: Belongs to the Pup ligase/Pup deamidase family. Pup-
CC       conjugating enzyme subfamily.
XX
DR   Pfam; PF03136; Pup_ligase; 1; trigger=no
DR   NCBIfam; TIGR03686; Pupylate_PafA; 1; trigger=no
DR   PIRSF; PIRSF018077; UCP018077; 1; trigger=no
XX
KW   ATP-binding
KW   Ligase
KW   Magnesium
KW   Metal-binding
KW   Nucleotide-binding
KW   Ubl conjugation pathway
XX
GO   GO:0000287; F:magnesium ion binding
GO   GO:0005524; F:ATP binding
GO   GO:0019787; F:ubiquitin-like protein transferase activity
GO   GO:0010498; P:proteasomal protein catabolic process
GO   GO:0019941; P:modification-dependent protein catabolic process
GO   GO:0070490; P:protein pupylation
XX
FT   From: PAFA_CORGL (Q8NQE1)
FT   ACT_SITE        64
FT                   /note="Proton acceptor"
FT   Condition: D
FT   BINDING         16
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: E
FT   BINDING         62
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: Y
FT   BINDING         70
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: E
FT   BINDING         60
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: R
FT   BINDING         73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: [TS]
FT   BINDING         440
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: W
XX
Size: 447-504;
Related: None;
Template: P9WNU7; A0QZ42; Q8NQE1;
Scope:
 Bacteria; Actinomycetota
Fusion:
 Nter: None
 Cter: None
Duplicate: in RHOER
Plasmid: None
Comments: None
XX
# Revision 1.18 2023/06/01
//