AC   MF_02213;
DC   Protein; auto
TR   HAMAP; MF_02213; -; 1; level=0
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Names: Lipid_II_synth_GatD
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ID   GATD
DE   RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD;
DE            EC=6.3.5.13;
DE   AltName: Full=Lipid II isoglutaminyl synthase glutaminase subunit;
DE            EC=3.5.1.2;
GN   Name=gatD;
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CC   -!- FUNCTION: The lipid II isoglutaminyl synthase complex catalyzes the
CC       formation of alpha-D-isoglutamine in the cell wall lipid II stem
CC       peptide. The GatD subunit catalyzes the hydrolysis of glutamine to
CC       glutamate and ammonia. The resulting ammonia molecule is channeled to
CC       the active site of MurT.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-
CC         D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-
CC         glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-
CC         isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:57928,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359,
CC         ChEBI:CHEBI:60033, ChEBI:CHEBI:62233, ChEBI:CHEBI:456216;
CC         EC=6.3.5.13;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC         Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBUNIT: Forms a heterodimer with MurT.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. GatD subfamily.
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DR   PROSITE; PS51274; GATASE_COBBQ; 1; trigger=yes
DR   Pfam; PF07685; GATase_3; 1; trigger=no
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KW   Cell shape
KW   Cell wall biogenesis/degradation
KW   Glutamine amidotransferase
KW   Hydrolase
KW   Ligase
KW   Peptidoglycan synthesis
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GO   GO:0140282; F:carbon-nitrogen ligase activity on lipid II
GO   GO:0004359; F:glutaminase activity
GO   GO:0009252; P:peptidoglycan biosynthetic process
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FT   From: GATD_STAAC (A0A0H2WZ38)
FT   ACT_SITE        94
FT                   /note="Nucleophile"
FT   Condition: C
FT   ACT_SITE        189
FT   Condition: H
FT   BINDING         128
FT                   /ligand="substrate"
FT   Condition: R
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Size: 210-390;
Related: None;
Template: A0A0H2WZ38; A0A0H3JN63; Q8DNZ8;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
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# Revision 1.5 2022/05/14
//