AC   MF_02221;
DC   Protein; auto
TR   HAMAP; MF_02221; -; 1; level=0
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Names: CPSase
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ID   CPS
DE   RecName: Full=Carbamoyl-phosphate synthase;
DE            EC=6.3.4.16;
DE   AltName: Full=Carbamoyl phosphate synthetase;
DE            Short=CPSase;
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CC   -!- FUNCTION: Catalyzes the synthesis of carbamoyl phosphate from ATP,
CC       ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the
CC       phosphorylation of hydrogencarbonate to carboxyphosphate, a
CC       nucleophilic attack of ammonia on carboxyphosphate yielding carbamate,
CC       and the phosphorylation of carbamate forming carbamoyl phosphate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl
CC         phosphate + 2 H(+) + phosphate; Xref=Rhea:RHEA:18029,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228,
CC         ChEBI:CHEBI:456216; EC=6.3.4.16;
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18030;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=Binds 2 magnesium or manganese ions per subunit
CC   -!- SIMILARITY: Belongs to the small carbamoyl-phosphate synthase family.
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DR   PROSITE; PS50975; ATP_GRASP; 1; trigger=yes
DR   Pfam; PF02655; ATP-grasp_3; 1; trigger=no
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KW   ATP-binding
KW   Ligase
KW   Magnesium
KW   Manganese
KW   Metal-binding
KW   Nucleotide-binding
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GO   GO:0005524; F:ATP binding
GO   GO:0004087; F:carbamoyl-phosphate synthase (ammonia) activity
GO   GO:0000287; F:magnesium ion binding
GO   GO:0030145; F:manganese ion binding
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FT   From: CPS_METS3 (A5UK38)
FT   BINDING         137..186
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
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Size: 338-480;
Related: None;
Template: A5UK38;
Scope:
 Archaea; Methanobacteria
 Archaea; Methanopyri
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
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# Revision 1.4 2022/11/19
//