AC   MF_03023;
DC   Protein; auto
TR   HAMAP; MF_03023; -; 1; level=0
XX
Names: Katanin_p60_A1
XX
ID   KTNA1
case <OC:Caenorhabditis>
DE   RecName: Full=Meiotic spindle formation protein mei-1;
DE            EC=5.6.1.1;
DE   AltName: Full=Katanin p60 ATPase-containing subunit A1;
DE            Short=Katanin p60 subunit A1;
DE   AltName: Full=p60 katanin;
GN   Name=mei-1;
else
DE   RecName: Full=Katanin p60 ATPase-containing subunit A1;
DE            Short=Katanin p60 subunit A1;
DE            EC=5.6.1.1;
DE   AltName: Full=p60 katanin;
GN   Name=KATNA1;
end case
XX
case <OC:Mammalia>
CC   -!- FUNCTION: Catalytic subunit of a complex which severs microtubules in
CC       an ATP-dependent manner. Microtubule severing may promote rapid
CC       reorganization of cellular microtubule arrays and the release of
CC       microtubules from the centrosome following nucleation. Microtubule
CC       release from the mitotic spindle poles may allow depolymerization of
CC       the microtubule end proximal to the spindle pole, leading to poleward
CC       microtubule flux and poleward motion of chromosome. Microtubule release
CC       within the cell body of neurons may be required for their transport
CC       into neuronal processes by microtubule-dependent motor proteins. This
CC       transport is required for axonal growth.
else case <OC:Caenorhabditis>
CC   -!- FUNCTION: Catalytic subunit of a complex which severs microtubules in
CC       an ATP-dependent manner. Microtubule severing may promote rapid
CC       reorganization of cellular microtubule arrays. This activity may
CC       promote rapid reorganization of cellular microtubule arrays. Required
CC       specifically for meiotic spindle formation in the female germline; the
CC       presence of this protein is inimical to the formation of mitotic
CC       spindles.
else case <OC:Metazoa>
CC   -!- FUNCTION: Catalytic subunit of a complex which severs microtubules in
CC       an ATP-dependent manner. Microtubule severing may promote rapid
CC       reorganization of cellular microtubule arrays and the release of
CC       microtubules from the centrosome following nucleation.
else
CC   -!- FUNCTION: Severs microtubules in an ATP-dependent manner. Microtubule
CC       severing may promote rapid reorganization of cellular microtubule
CC       arrays.
end case
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)
CC         alpha/beta tubulin heterodimers.; EC=5.6.1.1;
case <OC:Caenorhabditis>
CC   -!- ACTIVITY REGULATION: ATPase activity is stimulated by microtubules,
CC       which promote homooligomerization. ATP-dependent microtubule severing
CC       is stimulated by interaction with mei-2.
else case <OC:Metazoa>
CC   -!- ACTIVITY REGULATION: ATPase activity is stimulated by microtubules,
CC       which promote homooligomerization. ATP-dependent microtubule severing
CC       is stimulated by interaction with @gn(KATNB1).
end case
case <OC:Mammalia>
CC   -!- SUBUNIT: Can homooligomerize into hexameric rings, which may be
CC       promoted by interaction with microtubules. Interacts with KATNB1, which
CC       may serve as a targeting subunit. Interacts with ASPM; the katanin
CC       complex formation KATNA1:KATNB1 is required for the association of
CC       ASPM. Interacts with dynein and NDEL1. Associates with the E3 ligase
CC       complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP
CC       complex). Interacts with KLHL42 (via the kelch domains). Interacts with
CC       CUL3; the interaction is enhanced by KLHL42. Interacts with KATNB1 and
CC       KATNBL1. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the
CC       length of CAMSAP-decorated microtubule stretches.
else case <OC:Caenorhabditis>
CC   -!- SUBUNIT: Interacts with mei-2, which may serve as a targeting subunit.
CC       Also interacts with mel-26, which targets mei-1 for ubiquitin mediated
CC       proteolysis.
else case <OC:Metazoa>
CC   -!- SUBUNIT: Can homooligomerize into hexameric rings, which may be
CC       promoted by interaction with microtubules. Interacts with @gn(KATNB1),
CC       which may serve as a targeting subunit.
end case
case <OC:Mammalia>
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Midbody. Cytoplasm, cytoskeleton,
CC       microtubule organizing center, centrosome. Cytoplasm, cytoskeleton,
CC       spindle pole. Cytoplasm, cytoskeleton, spindle. Note=Predominantly
CC       cytoplasmic. Localized diffusely in the cytoplasm during the
CC       interphase. During metaphase is localized throughout the cell and more
CC       widely dispersed than the microtubules. In anaphase and telophase is
CC       localized at the midbody region. Also localized to the interphase
CC       centrosome and the mitotic spindle poles. Enhanced recruitment to the
CC       mitotic spindle poles requires microtubules and interaction with
CC       KATNB1. Localizes within the cytoplasm, partially overlapping with
CC       microtubules, in interphase and to the mitotic spindle and spindle
CC       poles during mitosis.
else case <OC:Caenorhabditis>
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole.
CC       Note=Localized to the spindle poles and condensed chromatin during
CC       female meiosis. This localization requires mei-2. Also localized to the
CC       polar body.
else case <OC:Metazoa>
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
CC       Cytoplasm, cytoskeleton, spindle. Note=Predominantly cytoplasmic. Also
CC       localized to the interphase centrosome and the mitotic spindle poles.
CC       Enhanced recruitment to the mitotic spindle poles requires microtubules
CC       and interaction with @gn(KATNB1).
else
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
end case
case <OC:Mammalia>
CC   -!- DOMAIN: The N-terminus is sufficient for interaction with microtubules,
CC       although high affinity binding to microtubules also requires an intact
CC       C-terminal domain and ATP, which promotes oligomerization.
CC   -!- PTM: Phosphorylation by DYRK2 triggers ubiquitination and subsequent
CC       degradation.
CC   -!- PTM: Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex,
CC       leading to its proteasomal degradation. Ubiquitinated by the EDVP E3
CC       ligase complex and subsequently targeted for proteasomal degradation.
end case
CC   -!- SIMILARITY: Belongs to the AAA ATPase family. Katanin p60 subunit A1
CC       subfamily.
XX
DR   PROSITE; PS00674; AAA; 1; trigger=no
DR   Pfam; PF00004; AAA; 1; trigger=no
XX
KW   ATP-binding
KW   Cytoplasm
KW   Cytoskeleton
KW   Isomerase
KW   Microtubule
KW   Nucleotide-binding
case <OC:Metazoa>
KW   Cell cycle
KW   Cell division
end case
case <OC:Caenorhabditis>
KW   Meiosis
else case <OC:Metazoa>
KW   Mitosis
end case
case <OC:Mammalia>
KW   Phosphoprotein
KW   Ubl conjugation
end case
XX
GO   GO:0008017; F:microtubule binding
GO   GO:0051013; P:microtubule severing
GO   GO:0005737; C:cytoplasm
case <OC:Metazoa>
GO   GO:0005813; C:centrosome
GO   GO:0005819; C:spindle
end case
XX
FT   From: KTNA1_HUMAN (O75449)
FT   BINDING         249..256
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: G-P-P-G-T-G-K-T
case <OC:Mammalia>
FT   MOD_RES         42
FT                   /note="Phosphoserine; by DYRK2"
FT   Condition: S
FT   MOD_RES         109
FT                   /note="Phosphoserine; by DYRK2"
FT   Condition: S
FT   MOD_RES         133
FT                   /note="Phosphothreonine; by DYRK2"
FT   Condition: T
end case
XX
Size: 464-681;
Related: None;
Template: O75449; O61577;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.22 2022/11/19
//