AC MF_03038; DC Protein; auto c? TR HAMAP; MF_03038; -; 1; level=0 XX Names: NUBP1 XX case or ID NBP35 else ID NUBP1 end case case DE RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP1; DE AltName: Full=Nucleotide-binding protein 1; DE Short=NBP 1; else case DE RecName: Full=Cytosolic Fe-S cluster assembly factor ; DE AltName: Full=Nucleotide-binding protein 1; DE Short=NBP 1; else case DE RecName: Full=Cytosolic Fe-S cluster assembly factor ; DE AltName: Full=Nucleotide-binding protein 35; else case DE RecName: Full=Cytosolic Fe-S cluster assembly factor NBP35; else DE RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP1 homolog; end case case GN Name=NUBP1; else case or GN Name=NBP35; end case XX case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold CC complex, mediating the de novo assembly of an Fe-S cluster and its CC transfer to target apoproteins. Required for biogenesis and export of CC both ribosomal subunits, which may reflect a role in assembly of the CC Fe/S clusters in RLI1, a protein which performs rRNA processing and CC ribosome export. else case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S CC scaffold complex, mediating the de novo assembly of an Fe-S cluster and CC its transfer to target apoproteins. else case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe-S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo CC assembly of an Fe-S cluster and its transfer to target apoproteins. CC Essential for embryo development. else case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S CC scaffold complex, mediating the de novo assembly of an Fe-S cluster and CC its transfer to target apoproteins. Implicated in the regulation of CC centrosome duplication. else CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S CC scaffold complex, mediating the de novo assembly of an Fe-S cluster and CC its transfer to target apoproteins. end case case CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable CC clusters in the N-termini and one labile, bridging cluster between CC subunits of the homodimer.; else case CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable CC clusters in the N-termini of @gn(NBP35) and two labile, bridging CC clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer.; else CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable CC clusters in the N-termini of @gn(NUBP1) and two labile, bridging CC clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer.; end case case CC -!- SUBUNIT: Homodimer and homotetramer. Predominantly homodimeric. else case CC -!- SUBUNIT: Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with CC KIFC1. else case CC -!- SUBUNIT: Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains. else CC -!- SUBUNIT: Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains. end case case CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. else CC -!- SUBCELLULAR LOCATION: Cytoplasm. end case CC -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family. CC NUBP1/NBP35 subfamily. case CC -!- MISCELLANEOUS: Although plant and algal NBP35 proteins lack the CC characteristic CXXC motif in the C-terminus, thought to be required for CC Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C- CC terminus. Also, in this linage, no CFD1 partner protein homolog as CC found in other eukaryotes can be found. end case XX DR PROSITE; PS01215; MRP; 1; trigger=no DR Pfam; PF10609; ParA; 1; trigger=no XX case or KW 4Fe-4S KW Iron KW Iron-sulfur KW Metal-binding end case case KW ATP-binding KW Nucleotide-binding end case KW Cytoplasm case KW Nucleus end case XX GO GO:0051539; F:4 iron, 4 sulfur cluster binding GO GO:0016226; P:iron-sulfur cluster assembly GO GO:0005737; C:cytoplasm XX FT From: NBP35_YEAST (P52920) FT BINDING 80..87 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-K-G-G-V-G-K-S FT BINDING 27 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 41 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 44 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C FT BINDING 50 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="1" FT Group: 1; Condition: C case not FT BINDING 253 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT /ligand_note="ligand shared with heterodimeric FT partner" FT Group: 2; Condition: C FT BINDING 256 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_label="2" FT /ligand_note="ligand shared with heterodimeric FT partner" FT Group: 2; Condition: C end case XX Size: 310-345; Related: MF_02040; Template: P53384; P52920; Q9R060; Q8H1Q2; Scope: Eukaryota Fusion: Nter: None Cter: None Duplicate: in XENLA Plasmid: None Comments: None XX # Revision 1.12 2022/11/19 //