AC MF_03039; DC Protein; auto c? TR HAMAP; MF_03039; -; 1; level=0 XX Names: NUBP2 XX case ID CFD1 else ID NUBP2 end case case DE RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP2; DE AltName: Full=Nucleotide-binding protein 2; DE Short=NBP 2; else case DE RecName: Full=Cytosolic Fe-S cluster assembly factor ; DE AltName: Full=Nucleotide-binding protein 2; DE Short=NBP 2; else case DE RecName: Full=Cytosolic Fe-S cluster assembly factor ; DE AltName: Full=Cytosolic Fe-S cluster-deficient protein 1; else DE RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP2 homolog; end case case GN Name=NUBP2; else case GN Name=CFD1; end case XX case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold CC complex, mediating the de novo assembly of an Fe-S cluster and its CC transfer to target apoproteins. Required for biogenesis and export of CC both ribosomal subunits, which may reflect a role in assembly of the CC Fe/S clusters in RLI1, a protein which performs rRNA processing and CC ribosome export. else case CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The @gn(NBP35)-@gn(CFD1) heterotetramer forms a Fe-S CC scaffold complex, mediating the de novo assembly of an Fe-S cluster and CC its transfer to target apoproteins. else CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein CC assembly (CIA) machinery. Required for maturation of extramitochondrial CC Fe-S proteins. The @gn(NUBP1)-@gn(NUBP2) heterotetramer forms a Fe-S CC scaffold complex, mediating the de novo assembly of an Fe-S cluster and CC its transfer to target apoproteins. end case case CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable CC clusters in the N-termini of @gn(NBP35) and two labile, bridging CC clusters between subunits of the @gn(NBP35)-@gn(CFD1) heterotetramer.; else CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable CC clusters in the N-termini of @gn(NUBP1) and two labile, bridging CC clusters between subunits of the @gn(NUBP1)-@gn(NUBP2) heterotetramer.; end case case CC -!- SUBUNIT: Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with CC KIFC1. else case CC -!- SUBUNIT: Heterotetramer of 2 @gn(NBP35) and 2 @gn(CFD1) chains. else CC -!- SUBUNIT: Heterotetramer of 2 @gn(NUBP1) and 2 @gn(NUBP2) chains. end case case CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule CC organizing center, centrosome. Note=Enriched at the centrosomes during CC mitosis. else CC -!- SUBCELLULAR LOCATION: Cytoplasm. end case CC -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family. CC NUBP2/CFD1 subfamily. XX DR PROSITE; PS01215; MRP; 1; trigger=no DR Pfam; PF10609; ParA; 1; trigger=no XX case KW Acetylation end case case KW 4Fe-4S KW Iron KW Iron-sulfur KW Metal-binding end case case KW ATP-binding KW Nucleotide-binding end case KW Cytoplasm case KW Cytoskeleton KW Nucleus end case XX GO GO:0051539; F:4 iron, 4 sulfur cluster binding GO GO:0016226; P:iron-sulfur cluster assembly XX FT From: CFD1_YEAST (P40558) FT BINDING 25..32 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-K-G-G-V-G-K-S FT BINDING 201 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_note="ligand shared between dimeric partners" FT Group: 1; Condition: C FT BINDING 204 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT /ligand_note="ligand shared between dimeric partners" FT Group: 1; Condition: C FT From: NUBP2_HUMAN (Q9Y5Y2) case FT MOD_RES 1 FT /note="N-acetylmethionine" FT Tag: acetyl; Condition: M end case XX Size: 255-361; Related: MF_02040; Template: Q9Y5Y2; Q9R061; P40558; Scope: Eukaryota Fusion: Nter: None Cter: MF_03037 Duplicate: in DROYA Plasmid: None Comments: None XX # Revision 1.11 2022/11/19 //