AC   MF_03048;
DC   Protein; auto
TR   HAMAP; MF_03048; -; 1; level=0
XX
Names: Urm1
XX
ID   URM1
case <OC:Fungi> or <OC:Vertebrata>
DE   RecName: Full=Ubiquitin-related modifier 1;
else
DE   RecName: Full=Ubiquitin-related modifier 1 homolog;
end case
case <OC:Fungi> or <OC:Vertebrata>
GN   Name=URM1;
else case <OC:Caenorhabditis>
GN   Name=urm-1;
else case <OC:Drosophilidae>
GN   Name=Urm1;
end case
XX
case <OC:Fungi>
CC   -!- FUNCTION: Acts as a sulfur carrier required for 2-thiolation of
CC       mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu)
CC       and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by
CC       being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog
CC       @gn(UBA4). The sulfur is then transferred to tRNA to form 2-thiolation
CC       of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex
CC       is required for 2-thiolation. Also acts as a ubiquitin-like protein
CC       (UBL) that is covalently conjugated via an isopeptide bond to lysine
CC       residues of target proteins such as @gn(AHP1). The thiocarboxylated
CC       form serves as substrate for conjugation and oxidative stress
CC       specifically induces the formation of UBL-protein conjugates.
else case <OC:Mammalia>
CC   -!- FUNCTION: Acts as a sulfur carrier required for 2-thiolation of
CC       mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu)
CC       and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by
CC       being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur
CC       is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also
CC       acts as a ubiquitin-like protein (UBL) that is covalently conjugated
CC       via an isopeptide bond to lysine residues of target proteins such as
CC       MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as
CC       substrate for conjugation and oxidative stress specifically induces the
CC       formation of UBL-protein conjugates.
else case <OC:Vertebrata> or <OC:Viridiplantae>
CC   -!- FUNCTION: Acts as a sulfur carrier required for 2-thiolation of
CC       mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu)
CC       and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by
CC       being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur
CC       is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also
CC       acts as a ubiquitin-like protein (UBL) that is covalently conjugated
CC       via an isopeptide bond to lysine residues of target proteins. The
CC       thiocarboxylated form serves as substrate for conjugation and oxidative
CC       stress specifically induces the formation of UBL-protein conjugates.
else
CC   -!- FUNCTION: Acts as a sulfur carrier required for 2-thiolation of
CC       mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu)
CC       and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by
CC       being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4
CC       homolog. The sulfur is then transferred to tRNA to form 2-thiolation of
CC       mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is
CC       covalently conjugated via an isopeptide bond to lysine residues of
CC       target proteins. The thiocarboxylated form serves as substrate for
CC       conjugation and oxidative stress specifically induces the formation of
CC       UBL-protein conjugates.
end case
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis.
case <OC:Mammalia>
CC   -!- SUBUNIT: Component of a complex at least composed of URM1, CTU2/NCS2
CC       and CTU1/ATPBD3.
else case <OC:Saccharomyces>
CC   -!- SUBUNIT: Homodimer; homodimerization may provide an autoprotection to
CC       the highly active C-terminal residue before attacking its substrates.
CC       Interacts with NCS2 and NCS6. Forms a conjugate with the target protein
CC       AHP1.
end case
case <OC:Saccharomyces>
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
else
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
end case
case <OC:Fungi>
CC   -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC       adenylated (-COAMP) via the hesA/moeB/thiF part of @gn(UBA4), then
CC       thiocarboxylated (-COSH) via the rhodanese domain of @gn(UBA4).
else case <OC:Vertebrata>
CC   -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC       adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then
CC       thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
else
CC   -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC       adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4
CC       homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the
CC       MOCS3/UBA4 homolog.
end case
CC   -!- SIMILARITY: Belongs to the URM1 family.
XX
DR   Pfam; PF09138; Urm1; 1; trigger=no
XX
KW   Cytoplasm
case <OC:Saccharomyces>
KW   Nucleus
end case
KW   Isopeptide bond
KW   tRNA processing
KW   Ubl conjugation pathway
XX
GO   GO:0005829; C:cytosol
case <OC:Saccharomyces>
GO   GO:0005634; C:nucleus
end case
GO   GO:0002098; P:tRNA wobble uridine modification
GO   GO:0032447; P:protein urmylation
GO   GO:0034227; P:tRNA thio-modification
XX
FT   From: URM1_YEAST (P40554)
FT   MOD_RES         99
FT                   /note="1-thioglycine"
FT   Condition: G
FT   CROSSLNK        99
FT                   /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT                   K-? in acceptor proteins)"
FT   Condition: G
XX
Size: 91-142;
Related: None;
Template: P40554; Q9BTM9;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: in ARATH, CANAL
Plasmid: None
Comments: None
XX
# Revision 1.7 2019/11/20
//