AC   MF_03053;
DC   Protein; auto
TR   HAMAP; MF_03053; -; 1; level=0
XX
Names: CTU1
XX
ID   CTU1
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1;
DE            EC=2.7.7.-;
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1;
case <OC:Vertebrata>
DE   + AltName: Full=ATP-binding domain-containing protein 3;
else case <OC:Caenorhabditis>
DE   + AltName: Full=Thiolation of uridine in tRNA protein 1;
else case <OC:Saccharomyces>
DE   + AltName: Full=Thiolation of uridine in cytoplasmic tRNA protein 1;
DE   + AltName: Full=Needs CLA4 to survive protein 6;
else
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1;
DE            EC=2.7.7.-;
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1;
end case
case <OC:Vertebrata>
GN   Name=CTU1; Synonyms=ATPBD3, NCS6;
else case <OC:Caenorhabditis>
GN   Name=tut-1; Synonyms=ctu-1;
else case <OC:Saccharomyces>
GN   Name=NCS6; Synonyms=CTU1, TUC1;
else case <OC:Fungi> or <OC:Viridiplantae>
GN   Name=NCS6; Synonyms=CTU1;
end case
XX
case <OC:Fungi>
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated
CC       @gn(URM1) onto the uridine of tRNAs at wobble position. Prior mcm(5)
CC       tRNA modification by the elongator complex is required for 2-
CC       thiolation. May also be involved in protein urmylation.
else
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated
CC       @gn(URM1) onto the uridine of tRNAs at wobble position.
end case
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis.
case <OC:Mammalia>
CC   -!- SUBUNIT: Component of a complex at least composed of URM1, CTU2/NCS2
CC       and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.
else case <OC:Saccharomyces>
CC   -!- SUBUNIT: Interacts with NCS2 and URM1. May act by forming a heterodimer
CC       with NCS2. Component of a large molecular weight complex of more than
CC       250 kDa.
end case
case <OC:Saccharomyces>
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion.
else
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
end case
CC   -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
XX
DR   PROSITE; PS01263; UPF0021; 1; trigger=no
DR   Pfam; PF01171; ATP_bind_3; 1; trigger=no
DR   NCBIfam; TIGR00269; UPF0021; 1; trigger=no
XX
KW   Cytoplasm
case <OC:Saccharomyces>
KW   Mitochondrion
end case
KW   RNA-binding
KW   Transferase
KW   tRNA processing
KW   tRNA-binding
XX
GO   GO:0000049; F:tRNA binding
GO   GO:0016779; F:nucleotidyltransferase activity
GO   GO:0002098; P:tRNA wobble uridine modification
GO   GO:0032447; P:protein urmylation
GO   GO:0034227; P:tRNA thio-modification
GO   GO:0005737; C:cytoplasm
XX
FT   None
XX
Size: 313-443;
Related: None;
Template: Q7Z7A3; P53088; O94282;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.5 2023/06/01
//