AC MF_03055; DC Protein; auto TR HAMAP; MF_03055; -; 1; level=0 XX Names: tRNA_methyltr_TrmB_euk XX ID TRMB DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase; DE EC=2.1.1.33; DE AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase; DE AltName: Full=tRNA(m7G46)-methyltransferase; case DE + AltName: Full=Methyltransferase-like protein 1; else case DE + AltName: Full=Transfer RNA methyltransferase 8; end case case or GN Name=METTL1; else case GN Name=metl-1; else case GN Name=TRM8; end case XX case CC -!- FUNCTION: Methyltransferase that catalyzes the formation of N(7)- CC methylguanine at position 46 (m7G46) in tRNA, a modification required CC to maintain stability of tRNAs; its absence resulting in tRNA decay. CC Both the D-stem and T-stem structures of tRNAs are required for CC efficient methyltransferase activity. else CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46 CC (m7G46) in tRNA. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- CC methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, CC ChEBI:CHEBI:74480; EC=2.1.1.33; CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. case CC -!- SUBUNIT: Forms a complex with @gn(WDR4). else case CC -!- SUBUNIT: Forms a complex with @gn(TRM82). end case CC -!- SUBCELLULAR LOCATION: Nucleus. case CC -!- PTM: Phosphorylation at #{Ser-27} inactivates its catalytic activity CC but does not affect the interaction with @gn(WDR4). end case CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase CC superfamily. TrmB family. XX DR PROSITE; PS51625; SAM_MT_TRMB; 1; trigger=no DR Pfam; PF02390; Methyltransf_4; 1; trigger=no DR NCBIfam; TIGR00091; CHP91; 1; trigger=no XX KW Methyltransferase KW Nucleus KW RNA-binding KW S-adenosyl-L-methionine KW Transferase KW tRNA processing KW tRNA-binding case KW Phosphoprotein end case XX GO GO:0000049; F:tRNA binding GO GO:0008176; F:tRNA (guanine(46)-N7)-methyltransferase activity GO GO:0005634; C:nucleus GO GO:0030488; P:tRNA methylation XX FT From: TRMB_HUMAN (Q9UBP6) FT BINDING 107..108 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: E-[IL] FT BINDING 140..141 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: N-x FT BINDING 238..240 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [ST]-[ED]-[ED] FT ACT_SITE 163 FT Condition: D FT BINDING 84 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: G FT BINDING 160 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [LC] case FT MOD_RES 27 FT /note="Phosphoserine; by PKB and RPS6KA3" FT Tag: phospho; Condition: S end case XX Size: 214-377; Related: None; Template: Q9UBP6; Q12009; Scope: Eukaryota Fusion: Nter: None Cter: None Duplicate: in XENTR Plasmid: None Comments: None XX # Revision 1.12 2023/10/13 //