AC   MF_03071;
DC   Protein; auto
TR   HAMAP; MF_03071; -; 1; level=0
XX
Names: CHD8
XX
ID   CHD8
DE   RecName: Full=Chromodomain-helicase-DNA-binding protein 8;
DE            Short=CHD-8;
DE            EC=3.6.4.12;
DE   AltName: Full=ATP-dependent helicase CHD8;
GN   Name=CHD8;
XX
case <OC:Mammalia>
CC   -!- FUNCTION: DNA helicase that acts as a chromatin remodeling factor and
CC       regulates transcription. Acts as a transcription repressor by
CC       remodeling chromatin structure and recruiting histone H1 to target
CC       genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1
CC       and preventing p53/TP53 transactivation activity. Acts as a negative
CC       regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1)
CC       activity. Negatively regulates CTNNB1-targeted gene expression by being
CC       recruited specifically to the promoter regions of several CTNNB1
CC       responsive genes. Involved in both enhancer blocking and epigenetic
CC       remodeling at chromatin boundary via its interaction with CTCF. Acts as
CC       a suppressor of STAT3 activity by suppressing the LIF-induced STAT3
CC       transcriptional activity. Also acts as a transcription activator via
CC       its interaction with ZNF143 by participating in efficient U6 RNA
CC       polymerase III transcription.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3.
CC       Component of some MLL1/MLL complex, at least composed of the core
CC       components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as
CC       the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1,
CC       LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2,
CC       RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and
CC       TEX10. Interacts with CHD7. Interacts with FAM124B.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Localizes to the promoter regions
CC       of several CTNNB1-responsive genes. Also present at known CTCF target
CC       sites.
CC   -!- PTM: Sumoylated.
else
CC   -!- FUNCTION: DNA helicase that acts as a chromatin remodeling factor and
CC       regulates transcription. Acts as a transcription repressor by
CC       remodeling chromatin structure and recruiting histone H1 to target
CC       genes. Suppresses p53/@gn(TP53)-mediated apoptosis by recruiting
CC       histone H1 and preventing p53/@gn(TP53) transactivation activity. Acts
CC       as a negative regulator of Wnt signaling pathway by regulating beta-
CC       catenin (@gn(CTNNB1)) activity. Negatively regulates @gn(CTNNB1)-
CC       targeted gene expression by being recruited specifically to the
CC       promoter regions of several @gn(CTNNB1) responsive genes. May also act
CC       as a transcription activator by participating in efficient U6 RNA
CC       polymerase III transcription.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Component of some MLL1/MLL complex.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
end case
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
XX
DR   PROSITE; PS00598; CHROMO_1; 2; trigger=no
DR   PROSITE; PS50013; CHROMO_2; 2; trigger=any
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1; trigger=no
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1; trigger=yes
DR   PROSITE; PS51194; HELICASE_CTER; 1; trigger=yes
DR   PROSITE; PS50312; ASP_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50322; GLN_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50324; SER_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50316; HIS_RICH; 0-unlimited; trigger=strict
DR   Pfam; PF07533; BRK; 1; trigger=no
DR   Pfam; PF00385; Chromo; 2; trigger=no
DR   Pfam; PF00271; Helicase_C; 1; trigger=no
DR   Pfam; PF00176; SNF2_N; 1; trigger=no
XX
KW   Activator
KW   ATP-binding
KW   Chromatin regulator
KW   DNA-binding
KW   Helicase
KW   Hydrolase
KW   Nucleotide-binding
KW   Nucleus
KW   Repeat
KW   Repressor
KW   Transcription
KW   Transcription regulation
KW   Wnt signaling pathway
case <FTTag:sumo>
KW   Isopeptide bond
KW   Ubl conjugation
end case
XX
GO   GO:0005634; C:nucleus
GO   GO:0071339; C:MLL1 complex
GO   GO:0005524; F:ATP binding
GO   GO:0003677; F:DNA binding
GO   GO:0003678; F:DNA helicase activity
GO   GO:0008094; F:ATP-dependent activity, acting on DNA
GO   GO:0002039; F:p53 binding
GO   GO:0042393; F:histone binding
GO   GO:0008013; F:beta-catenin binding
GO   GO:0006338; P:chromatin remodeling
GO   GO:0045893; P:positive regulation of DNA-templated transcription
GO   GO:0045892; P:negative regulation of DNA-templated transcription
GO   GO:0030178; P:negative regulation of Wnt signaling pathway
GO   GO:0043066; P:negative regulation of apoptotic process
XX
FT   From: CHD8_HUMAN (Q9HCK8)
case <OC:Mammalia>
FT   REGION          1789..2302
FT                   /note="Interaction with FAM124B"
end case
FT   From: CHD8_RAT (Q9JIX5)
case <OC:Mammalia>
FT   CROSSLNK        609
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO)"
FT   Tag: sumo; Condition: K
end case
XX
Size: 2184-2594;
Related: None;
Template: Q9HCK8; Q09XV5; Q9JIX5;
Scope:
 Eukaryota; Vertebrata
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.10 2022/06/27
//