AC   MF_03071;
DC   Protein; auto
TR   HAMAP; MF_03071; -; 1; level=0
XX
Names: CHD8
XX
ID   CHD8
DE   RecName: Full=Chromodomain-helicase-DNA-binding protein 8;
DE            Short=CHD-8;
DE            EC=3.6.4.-;
DE   AltName: Full=ATP-dependent helicase CHD8;
GN   Name=CHD8;
XX
case <OC:Mammalia>
CC   -!- FUNCTION: ATP-dependent chromatin-remodeling factor, it slides
CC       nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a
CC       transcription repressor by remodeling chromatin structure and
CC       recruiting histone H1 to target genes. Suppresses p53/TP53-mediated
CC       apoptosis by recruiting histone H1 and preventing p53/TP53
CC       transactivation activity. Acts as a negative regulator of Wnt signaling
CC       pathway by regulating beta-catenin (CTNNB1) activity. Negatively
CC       regulates CTNNB1-targeted gene expression by being recruited
CC       specifically to the promoter regions of several CTNNB1 responsive
CC       genes. Involved in both enhancer blocking and epigenetic remodeling at
CC       chromatin boundary via its interaction with CTCF. Acts as a suppressor
CC       of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional
CC       activity. Also acts as a transcription activator via its interaction
CC       with ZNF143 by participating in efficient U6 RNA polymerase III
CC       transcription.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC   -!- SUBUNIT: Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3.
CC       Component of some MLL1/MLL complex, at least composed of the core
CC       components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as
CC       the facultative components BACC1, CHD8, E2F6, HSP70, INO80C, KANSL1,
CC       LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2,
CC       RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and
CC       TEX10. Interacts with CHD7. Interacts with FAM124B.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Localizes to the promoter regions
CC       of several CTNNB1-responsive genes. Also present at known CTCF target
CC       sites.
CC   -!- PTM: Sumoylated.
else
CC   -!- FUNCTION: ATP-dependent chromatin-remodeling factor, it slides
CC       nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a
CC       transcription repressor by remodeling chromatin structure and
CC       recruiting histone H1 to target genes. Suppresses p53/@gn(TP53)-
CC       mediated apoptosis by recruiting histone H1 and preventing
CC       p53/@gn(TP53) transactivation activity. Acts as a negative regulator of
CC       Wnt signaling pathway by regulating beta-catenin (@gn(CTNNB1))
CC       activity. Negatively regulates @gn(CTNNB1)-targeted gene expression by
CC       being recruited specifically to the promoter regions of several
CC       @gn(CTNNB1) responsive genes. May also act as a transcription activator
CC       by participating in efficient U6 RNA polymerase III transcription.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC   -!- SUBUNIT: Component of some MLL1/MLL complex.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
end case
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
XX
DR   PROSITE; PS00598; CHROMO_1; 2; trigger=no
DR   PROSITE; PS50013; CHROMO_2; 2; trigger=any
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1; trigger=no
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1; trigger=yes
DR   PROSITE; PS51194; HELICASE_CTER; 1; trigger=yes
DR   PROSITE; PS50312; ASP_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50322; GLN_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50324; SER_RICH; 0-unlimited; trigger=strict
DR   PROSITE; PS50316; HIS_RICH; 0-unlimited; trigger=strict
DR   Pfam; PF07533; BRK; 1; trigger=no
DR   Pfam; PF00385; Chromo; 2; trigger=no
DR   Pfam; PF00271; Helicase_C; 1; trigger=no
DR   Pfam; PF00176; SNF2_N; 1; trigger=no
XX
KW   Activator
KW   ATP-binding
KW   Chromatin regulator
KW   DNA-binding
KW   Helicase
KW   Hydrolase
KW   Nucleotide-binding
KW   Nucleus
KW   Repeat
KW   Repressor
KW   Transcription
KW   Transcription regulation
KW   Wnt signaling pathway
case <FTTag:sumo>
KW   Isopeptide bond
KW   Ubl conjugation
end case
XX
GO   GO:0005634; C:nucleus
GO   GO:0071339; C:MLL1 complex
GO   GO:0005524; F:ATP binding
GO   GO:0003677; F:DNA binding
GO   GO:0003678; F:DNA helicase activity
GO   GO:0008094; F:ATP-dependent activity, acting on DNA
GO   GO:0002039; F:p53 binding
GO   GO:0042393; F:histone binding
GO   GO:0008013; F:beta-catenin binding
GO   GO:0006338; P:chromatin remodeling
GO   GO:0016887; F:ATP hydrolysis activity
GO   GO:0045893; P:positive regulation of DNA-templated transcription
GO   GO:0045892; P:negative regulation of DNA-templated transcription
GO   GO:0030178; P:negative regulation of Wnt signaling pathway
GO   GO:0043066; P:negative regulation of apoptotic process
XX
FT   From: CHD8_HUMAN (Q9HCK8)
case <OC:Mammalia>
FT   REGION          1789..2302
FT                   /note="Interaction with FAM124B"
end case
FT   From: CHD8_RAT (Q9JIX5)
case <OC:Mammalia>
FT   CROSSLNK        609
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO)"
FT   Tag: sumo; Condition: K
end case
XX
Size: 2184-2594;
Related: None;
Template: Q9HCK8; Q09XV5; Q9JIX5;
Scope:
 Eukaryota; Vertebrata
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Version: 15
# Last updated date: 2025-03-18
# Created date: 2016-08-03
//