AC   MF_03110;
DC   Protein; auto
TR   HAMAP; MF_03110; -; 1; level=0
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Names: Endonuc_su_Slx4
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ID   SLX4
DE   RecName: Full=Structure-specific endonuclease subunit @gn(SLX4);
GN   Name=SLX4;
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case <OC:Saccharomyces>
CC   -!- FUNCTION: Regulatory subunit that interacts with and increases the
CC       activity of different structure-specific endonucleases. Has several
CC       distinct roles in protecting genome stability by resolving diverse
CC       forms of deleterious DNA structures. Component of the SLX1-SLX4
CC       structure-specific endonuclease that resolves DNA secondary structures
CC       generated during DNA repair and recombination. Has endonuclease
CC       activity towards branched DNA substrates, introducing single-strand
CC       cuts in duplex DNA close to junctions with ss-DNA. Has a preference for
CC       simple Y, 5'-flap and replication fork-like structures. It cleaves the
CC       strand bearing the 5'-non-homologous arm at the branch site junction
CC       and generates ligatable, nicked products from the 5'-flap or
CC       replication fork substrates. Plays a critical role in maintaining the
CC       integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-
CC       starting stalled replication forks. Has Holliday junction resolvase
CC       activity in vitro. Interacts with the structure-specific RAD1-RAD10
CC       endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail
CC       removal (NHTR) during repair of double-strand breaks by single-strand
CC       annealing. SLX4 also promotes recovery from DNA-alkylation-induced
CC       replisome stalling during DNA replication by facilitating the error-
CC       free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10,
CC       but probably RTT107.
CC   -!- SUBUNIT: Forms a heterodimer with SLX1. Interacts with RAD1; catalytic
CC       subunit of the RAD1-RAD10 endonuclease. Interacts with RTT107.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
CC   -!- PTM: Phosphorylated by ATR (MEC1) and ATM (TEL1) upon DNA damage. This
CC       appears to be required for the function with the RAD1-RAD10
CC       endonuclease.
else case <OC:Fungi> and not <OC:Saccharomyces>
CC   -!- FUNCTION: Regulatory subunit of the @gn(SLX1)-@gn(SLX4) structure-
CC       specific endonuclease that resolves DNA secondary structures generated
CC       during DNA repair and recombination. Has endonuclease activity towards
CC       branched DNA substrates, introducing single-strand cuts in duplex DNA
CC       close to junctions with ss-DNA.
CC   -!- SUBUNIT: Forms a heterodimer with @gn(SLX1).
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- PTM: Phosphorylated in response to DNA damage.
end case
CC   -!- SIMILARITY: Belongs to the SLX4 family.
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DR   Pfam; PF09494; Slx4; 1; trigger=no
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KW   DNA damage
KW   DNA recombination
KW   DNA repair
KW   Nucleus
KW   Phosphoprotein
case <OC:Saccharomyces>
KW   Cytoplasm
end case
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GO   GO:0005634; C:nucleus
GO   GO:0033557; C:Slx1-Slx4 complex
GO   GO:0006310; P:DNA recombination
GO   GO:0006281; P:DNA repair
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FT   None
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Size: 419-1311;
Related: None;
Template: Q12098; Q9P6M0;
Scope:
 Eukaryota; Fungi
Fusion:
 Nter: None
 Cter: None
Duplicate: in CANTT
Plasmid: None
Comments: None
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# Revision 1.4 2019/11/20
//